Diagenode

DNA precipitant

カタログ番号
フォーマット
価格
C03030002
(kch-217-001)
1 ml
$30.00



This IP grade product is used for the precipitation of DNA after chromatin immunoprecipitation (ChIP) and after methylated DNA immunoprecipitation (Methyl DNA IP). It is included in our Transcription ChIP KIT (Cat. No. kch-redTBP-012), in our Histone ChIP KIT (Cat. No. kch-orgHIS-012) and in our Methylated DNA Immunoprecipitation KIT - MeDIP (Cat. No. mc-green-003). It is now separately available. Following IP and washes, the immunoprecipitated (IP’d) material is submitted to Phenol/Chloroform/Isomanyl alcohol (PCI) extraction. Then, to the aqueous phase obtained after PCI extraction of the IP’d material, our DNA precipitant is added with IP grade DNA co-precipitant (Cat. No. kch-216-100) and icecold 96% ethanol. For more details about these kits, see instruction manual.

  •  実験手法
    ChIP-qPCR
    定量のPCRとクロマチン免疫沈降(ChIP)が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、およ... Read more
  •  資料
    Datasheet DNA precipitant C03030002 DATASHEET
    Datasheet description
    Download
  •  Safety sheets
    DNA precipitant SDS GB en Download
    DNA precipitant SDS US en Download
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  •  出版物

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  •  関連商品

イベント

  • Virtual ChIP workshop - January 18-19, 2022 or January 25-26, 2022
    Diagenode
    Jan 18-Jan 26, 2022
  • Virtual DNA Methylation - January 18-19, 2022 or January 25-26, 2022
    Diagenode
    Jan 18-Jan 26, 2022
  • Lausanne Genomics Days 2022
    Lausanne, Switzerland
    Feb 14-Feb 15, 2022
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