CRBN monoclonal antibody

Catalog Number
10 μg
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Monoclonal antibody raised in mouse against the N-terminus of the CRBN (Cereblon). Diagenode Cereblon antibody, in contrast to most antibodies on the market, possesses excellent imunoprecipitating properties (see validation data).

Concentration2 µg/µl
Species reactivityHuman: positive. Other species: not tested.
PurityProtein G purified monoclonal antibody.
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferTBS containing 0.05 % Na-azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Western blotting 1:500 - :1,000 Fig 1
IP 1:200 Fig 2
  • Validation data

    CRBN Antibody validated in Western Blot

    Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN
    Western blot was performed on 20 µg protein extracts from wild type MOLT4 cells (lane 1) or MOLT4-CRBN-/- cells (lane 2) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:500 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.

    CRBN Antibody validated in Immunoprecipitation

    Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN
    IP was performed on whole cell extracts (400 µg) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 µg) is shown lane 1 and 2.

  • Target Description

    CRBN (UniProt/Swiss-Prot entry Q96SW2) is a cytoplasmic protease that is related to the Lon protease protein family. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. CRBN is associated with a calcium channel membrane protein and is thought to play a role in brain development and in memory and learning by regulating the assembly and neuronal surface expression of largeconductance calcium-activated potassium channels brain through its interaction with KCNT1. Mutations in the CRBN gene are associated with autosomal recessive nonsyndromic cognitive disability.

  •  実験手法
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunoprecipitation Read more
  •  資料
    CRBN monoclonal antibody datasheet DATASHEET
    Monoclonal antibody raised in mouse against the N-terminus of the CRBN (Cereblon).
  •  Safety sheets
    CRBN antibody SDS GB en Download
    CRBN antibody SDS US en Download
    CRBN antibody SDS BE nl Download
    CRBN antibody SDS FR fr Download
    CRBN antibody SDS BE fr Download
    CRBN antibody SDS ES es Download
    CRBN antibody SDS DE de Download
    CRBN antibody SDS JP ja Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: CRBN monoclonal antibody (Diagenode Cat# C15200245-10 Lot# 002). Click here to copy to clipboard.

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