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Monoclonal antibody raised in mouse against the N-terminus of the CRBN (Cereblon). Diagenode Cereblon antibody, in contrast to most antibodies on the market, possesses excellent imunoprecipitating properties (see validation data).
Human: positive. Other species: not tested.
Protein G purified monoclonal antibody.
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
TBS containing 0.02 % Na-azide.
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN Western blot was performed on protein extracts from wild type MOLT4 cells (lane 2) or MOLT4-CRBN-/- cells (lane 1) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:300 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.
Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN IP was performed on whole cell extracts (400 µg) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 µg) is shown lane 1 and 2.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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