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C2C2 polyclonal antibody (sample size)

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Catalog Number

Format

Price

C15310267-20

20 µl

$130.00
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C15310267-100

100 μl

Polyclonal antibody raised in rabbit against the class 2 type VI CRISPR-Cas effector (C2C2) Leptotrichia shahii using a recombinant protein.

Lot	A2915-004
Concentration	Not determined
Species reactivity	Leptotrichia shahii
Type	Polyclonal
Purity	Whole antiserum from rabbit containing 0.05% azide.
Host	Rabbit
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western Blotting	1:1,000 - 1/10,000	Fig 1

Validation data

Figure 1. Western blot analysis using the Diagenode antibody directed against C2C2
Western blot was performed on protein extracts from U2OS cells (lane 1) and on extracts from U2OS cells spiked with 50 ng of the immunogen (Lsh C2C2 recombinant protein, lane 2) using the Diagenode antibody against C2C2 (Cat. No. C15310267), diluted 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the C2C2 protein is indicated on the right. It’s unclear what the higher MW signal represents but since it is absent in the lane without the spiked protein, it might be a protein aggregate.

資料
C2C2 polyclonal antibody DATASHEET
Polyclonal antibody raised in rabbit against the class 2 type VI CRISPR-Cas effector (C2C2) Lepto... Download

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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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			'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified in <em>Leptotrichia shahii</em>. This CRISPR system is characterized by the presence of the single effector protein C2C2. C2C2 lacks homology to any known DNA nuclease domain but contains two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and is thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against C2C2</strong><br /> Western blot was performed on protein extracts from U2OS cells (lane 1) and on extracts from U2OS cells spiked with 50 ng of the immunogen (Lsh C2C2 recombinant protein, lane 2) using the Diagenode antibody against C2C2 (Cat. No. C15310267), diluted 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the C2C2 protein is indicated on the right. It&rsquo;s unclear what the higher MW signal represents but since it is absent in the lane without the spiked protein, it might be a protein aggregate.</small></p>
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		'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified in <em>Leptotrichia shahii</em>. This CRISPR system is characterized by the presence of the single effector protein C2C2. C2C2 lacks homology to any known DNA nuclease domain but contains two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and is thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15310267-wb.jpg" alt="Western blot figure 1" style="display: block; margin-left: auto; margin-right: auto;" width="272" height="350" /></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against C2C2</strong><br /> Western blot was performed on protein extracts from U2OS cells (lane 1) and on extracts from U2OS cells spiked with 50 ng of the immunogen (Lsh C2C2 recombinant protein, lane 2) using the Diagenode antibody against C2C2 (Cat. No. C15310267), diluted 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the C2C2 protein is indicated on the right. It&rsquo;s unclear what the higher MW signal represents but since it is absent in the lane without the spiked protein, it might be a protein aggregate.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against C2C2</strong><br /> Western blot was performed on protein extracts from U2OS cells (lane 1) and on extracts from U2OS cells spiked with 50 ng of the immunogen (Lsh C2C2 recombinant protein, lane 2) using the Diagenode antibody against C2C2 (Cat. No. C15310267), diluted 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the C2C2 protein is indicated on the right. It&rsquo;s unclear what the higher MW signal represents but since it is absent in the lane without the spiked protein, it might be a protein aggregate.</small></p>
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	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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