The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.
| Lot | 003D |
|---|---|
| Concentration | not determined |
| Species reactivity | Specific for blue colour used in protein MW markers |
| Type | Monoclonal |
| Purity | Protein A purified |
|---|---|
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Western Blotting | 1:1,000 - 1:5,000 | Fig 1, 2, 3 |
Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody
PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 μl of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol.
Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody
5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis.
Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody
Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested.
 How to properly cite this product in your workDiagenode strongly recommends using this: Blue ladder - HRP monoclonal antibody (Diagenode Cat# C15200202-100 Lot# 003D). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Anti-RAINBOW dye-specific antibodies as universal tools for the visualization of prestained protein molecular weight markers in Western blot analysis |
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