The new Blue ladder - HRP monoclonal antibody allows you to develop your ladder simultaneously with your proteins of interest during western blotting. Simply add this antibody along with your secondary HRP-coupled antibody and visualize your proteins and the blue pre-stained protein ladder on one membrane.
| Lot | 003D |
|---|---|
| Concentration | not determined |
| Species reactivity | Specific for blue colour used in protein MW markers |
| Type | Monoclonal |
| Purity | Protein A purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Western Blotting | 1:1,000 - 1:5,000 | Fig 1, 2, 3 |
Figure 1. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody
PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, Cat. No. 161-0373). 4 μl of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol.
Figure 2. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody
5 μl of the Page Ruler Plus Prestained Protein Ladder (Thermo Scientific, cat. No. 26619) were loaded on a gel and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C1520202) used at different dilutions. Figure 2 shows that even with a 1:5,000 dilution all blue prestained bands of the molecular weight marker are clearly visible after a 30” exposure, a typical exposure time for WB analysis.
Figure 3. Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody
Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested.
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Anti-RAINBOW dye-specific antibodies as universal tools for the visualization of prestained protein molecular weight markers in Western blot analysis |
