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Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against acrIIA4 Western blot was performed on protein extracts from U2OS cells spiked with the full length recombinant acrIIA4 protein (lane 1), using the Diagenode antibody against acrIIA4 (Cat. No. C15200248), diluted 1:1,000 in PBS-T containing 0.5% NFDM. Lane 2 shows the result of U2OS protein extracts only, used as negative control. The marker is shown on the left, the position of the acrIIA4 protein is indicated on the right.