Diagenode

The SAM domain-containing protein 1 (SAMD1) acts as a repressivechromatin regulator at unmethylated CpG islands


Stielow B. et al.

CpG islands (CGIs) are key regulatory DNA elements at most promoters, but how they influence the chromatin status and transcription remains elusive. Here, we identify and characterize SAMD1 (SAM domain-containing protein 1) as an unmethylated CGI-binding protein. SAMD1 has an atypical winged-helix domain that directly recognizes unmethylated CpG-containing DNA via simultaneous interactions with both the major and the minor groove. The SAM domain interacts with L3MBTL3, but it can also homopolymerize into a closed pentameric ring. At a genome-wide level, SAMD1 localizes to H3K4me3-decorated CGIs, where it acts as a repressor. SAMD1 tethers L3MBTL3 to chromatin and interacts with the KDM1A histone demethylase complex to modulate H3K4me2 and H3K4me3 levels at CGIs, thereby providing a mechanism for SAMD1-mediated transcriptional repression. The absence of SAMD1 impairs ES cell differentiation processes, leading to misregulation of key biological pathways. Together, our work establishes SAMD1 as a newly identified chromatin regulator acting at unmethylated CGIs.

Tags
Antibody
Microplex Library Preparation kit

Share this article

Published
May, 2021

Source

Products used in this publication

  • ChIP-seq Grade
    C15410039-50
    EZH2 Antibody - ChIP-seq Grade
  • cut and tag antibody icon
    C15410195
    H3K27me3 Antibody - ChIP-seq Grade
  • cut and tag antibody icon
    C15410035
    H3K4me2 Antibody - ChIP-seq Grade
  • cut and tag antibody icon
    C15410003-50
    H3K4me3 Antibody - ChIP-seq Grade
  • Mouse IgG
    C15410206
    Rabbit IgG
  • ChIP kit icon
    C05010012
    MicroPlex Library Preparation Kit v2 (12 indexes)
  • chip kit icon
    C05010001
    MicroPlex Library Preparation Kit v3 /48 rxns

活动

  • Long-Read Sequencing Meeting 2024
    Uppsala, Sweden
    Oct 21-Oct 23, 2024
  • NextGen Omics 2024
    London, UK
    Oct 23-Oct 25, 2024
  • FEBS 2024
    Budapest, Hungary
    Oct 28-Oct 31, 2024
  • 5th Danube Conference on Epigenetics
    Budapest, Hungary
    Oct 28-Oct 31, 2024
 查看所有活动

 


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy