The H6-GST-MBD fusion protein can be used to specifically isolate DNA containing methylated CpGs. It consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. *Number of cells that can be lysed and sheared with this kit.
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Prenatal Exposure to DEHP Affects Spermatogenesis and Sperm DNA Methylation in a Strain-Dependent Manner. Prados J, Stenz L, Somm E, Stouder C, Dayer A, Paoloni-Giacobino A Di-(2-ethylhexyl)phtalate (DEHP) is a plasticizer with endocrine disrupting properties found ubiquitously in the environment and altering reproduction in rodents. Here we investigated the impact of prenatal exposure to DEHP on spermatogenesis and DNA sperm methylation in two distinct, selected, and sequenced mice st...
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