Diagenode

Sequence features accurately predict genome-wide MeCP2 binding in vivo


Rube HT, Lee W, Hejna M, Chen H, Yasui DH, Hess JF, LaSalle JM, Song JS, Gong Q

Methyl-CpG binding protein 2 (MeCP2) is critical for proper brain development and expressed at near-histone levels in neurons, but the mechanism of its genomic localization remains poorly understood. Using high-resolution MeCP2-binding data, we show that DNA sequence features alone can predict binding with 88% accuracy. Integrating MeCP2 binding and DNA methylation in a probabilistic graphical model, we demonstrate that previously reported genome-wide association with methylation is in part due to MeCP2’s affinity to GC-rich chromatin, a result replicated using published data. Furthermore, MeCP2 co-localizes with nucleosomes. Finally, MeCP2 binding downstream of promoters correlates with increased expression in Mecp2-deficient neurons.

Tags
Antibody

Share this article

Published
March, 2016

Source

Products used in this publication

  • Antibody ChIP icon
    C15410052
    MeCP2 polyclonal antibody

イベント

  • Symposium: "Signaling through Chromatin"
    Grenoble, France
    Oct 2-Oct 4, 2023
  • EMBL Symposium: The non-coding genome
    Heidelberg, Germany
    Oct 11-Oct 14, 2023
  • IUBMB
    Crete, Greece
    Oct 15-Oct 20, 2023
 すべてのイベントを見る

ニュース

 すべてのニュースを見る


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics