Diagenode

Sequencing-based large-scale genomics approaches with small numbers of isolated maize meiocytes.


Dukowic-Schulze S, Sundararajan A, Ramaraj T, Mudge J, Chen C

High-throughput sequencing has become the large-scale approach of choice to study global gene expression and the distribution of specific chromatin marks and features. However, the limited availability of large amounts of purified cells made it very challenging to apply sequencing-based techniques in plant meiosis research in the past. In this paper, we describe a method to isolate meiocytes from maize anthers and detailed protocols to successfully perform RNA-seq, smRNA-seq, H3K4me3-ChIP-seq, and DNA bisulfite conversion sequencing with 5000-30,000 isolated maize male meiotic cells. These methods can be adjusted for other flowering plant species as well.

Tags
Bioruptor
Chromatin Shearing
ChIP-seq

Share this article

Published
February, 2014

Source

イベント

  • EpiChrom
    Umea Sweden
    Feb 27-Feb 28, 2020
 すべてのイベントを見る

ニュース

 すべてのニュースを見る


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics