The “NEBNext ChIP-Seq Illumina” protocol on the IP-Star® is using the standard “NEBNext® ChIP-Seq Library Prep for Illumina” kit and reagents from NEB®. This kit is compatible with Illumina® sequencers.
It provides flexibility to prepare 1 to 16 libraries in one run starting with 5-10ng of DNA. The whole protocol takes approximately 3h40 for 8 samples. It allows you to prepare up to 32 libraries per day with 2 runs.
At the end, you recover ligated products ready for size selection (if required) and amplification.
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