Spatial tissue proteomics of FFPE specimen using the Bioruptor® for sample preparation
Thanks to the excellent preservation of morphological features, formalin fixed and paraffin embedded (FFPE) tissues are an invaluable resource for spatial tissue proteomics. At the same time, due to the chemical crosslinking, it is extremely difficult to lyse such samples and solubilize proteins for deep proteomic analysis. A team of researchers from the EMBL in Heidelberg led by Martin Beck, Stephan Singer (University Hospital Heidelberg and University of Greifswald) and Alessandro Ori (Leibniz Institute on Aging – Fritz Lipmann Institute) has developed a strategy that utilizes the Bioruptor Plus in combination with heat-induced reversing of formalin fixation to enhance the efficient protein extraction from FFPE tissues . This enables the ultrasensitive and highly reproducible proteomic analysis of microdissected FFPE specimen with high spatial resolution.
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