Diagenode

Spatial tissue proteomics of FFPE specimen using the Bioruptor® for sample preparation

Thanks to the excellent preservation of morphological features, formalin fixed and paraffin embedded (FFPE) tissues are an invaluable resource for spatial tissue proteomics. At the same time, due to the chemical crosslinking, it is extremely difficult to lyse such samples and solubilize proteins for deep proteomic analysis. A team of researchers from the EMBL in Heidelberg led by Martin Beck, Stephan Singer (University Hospital Heidelberg and University of Greifswald) and Alessandro Ori (Leibniz Institute on Aging – Fritz Lipmann Institute) has developed a strategy that utilizes the Bioruptor Plus in combination with heat-induced reversing of formalin fixation to enhance the efficient protein extraction from FFPE tissues [1]. This enables the ultrasensitive and highly reproducible proteomic analysis of microdissected FFPE specimen with high spatial resolution.

関連商品

  • Bioruptor Plus Sonication Device
    B01020001
    Bioruptor® Plus sonication device

イベント

  • Long-Read Sequencing Meeting 2024
    Uppsala, Sweden
    Oct 21-Oct 23, 2024
  • NextGen Omics 2024
    London, UK
    Oct 23-Oct 25, 2024
  • FEBS 2024
    Budapest, Hungary
    Oct 28-Oct 31, 2024
  • 5th Danube Conference on Epigenetics
    Budapest, Hungary
    Oct 28-Oct 31, 2024
 すべてのイベントを見る

 


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy