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Next-generation sequencing library preparation using the Bioruptor. Shearing of both gDNA and dscDNA.

Shearing of DNA molecules is often required to obtain properly sized substrates for production of genome- wide libraries for sequencing by next-generation high- throughput deep-sequencing technologies. Using the Bioruptor, we have demonstrated that appropriately sized DNA molecules can be obtained in more than sufficient quantities for production of a number of different libraries for sequencing by next-generation sequencing technologies.

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