Diagenode

RUNX1 colludes with NOTCH1 to reprogram chromatin in T-cell acutelymphoblastic leukemia


Islam R. et al.

Runt-related transcription factor 1 (RUNX1) is oncogenic in diverse types of leukemia and epithelial cancers where its expression is associated with poor prognosis. Current models suggest that RUNX1 cooperates with other oncogenic factors (e.g., NOTCH1, TAL1) to drive the expression of proto-oncogenes in T cell acute lymphoblastic leukemia (T-ALL) but the molecular mechanisms controlled by RUNX1 and its cooperation with other factors remain unclear. Integrative chromatin and transcriptional analysis following inhibition of RUNX1 and NOTCH1 revealed a surprisingly widespread role of RUNX1 in the establishment of global H3K27ac levels and that RUNX1 is required by NOTCH1 for cooperative transcription activation of key NOTCH1 target genes including MYC, DTX1, HES4, IL7R, and NOTCH3. Super-enhancers were preferentially sensitive to RUNX1 knockdown and RUNX1-dependent super-enhancers were disrupted following the treatment of a pan-BET inhibitor, I-BET151.

Tags
Antibody

Share this article

Published
May, 2023

Source

Products used in this publication

  • cut and tag antibody icon
    C15410195
    H3K27me3 Antibody - ChIP-seq Grade
  • ChIP-seq Grade
    C15410037-50
    H3K4me1 Antibody - ChIP-seq Grade
  • ChIP-seq Grade
    C15410192
    H3K36me3 Antibody - ChIP-seq Grade
  • ChIP-seq Grade
    C15410056
    H3K9me3 Antibody - ChIP-seq Grade

 


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy