Diagenode

H19 lncRNA controls gene expression of the Imprinted Gene Network by recruiting MBD1.


Monnier P, Martinet C, Pontis J, Stancheva I, Ait-Si-Ali S, Dandolo L

The H19 gene controls the expression of several genes within the Imprinted Gene Network (IGN), involved in growth control of the embryo. However, the underlying mechanisms of this control remain elusive. Here, we identified the methyl-CpG-binding domain protein 1 MBD1 as a physical and functional partner of the H19 long noncoding RNA (lncRNA). The H19 lncRNA-MBD1 complex is required for the control of five genes of the IGN. For three of these genes-Igf2 (insulin-like growth factor 2), Slc38a4 (solute carrier family 38 member 4), and Peg1 (paternally expressed gene 1)-both MBD1 and H3K9me3 binding were detected on their differentially methylated regions. The H19 lncRNA-MBD1 complex, through its interaction with histone lysine methyltransferases, therefore acts by bringing repressive histone marks on the differentially methylated regions of these three direct targets of the H19 gene. Our data suggest that, besides the differential DNA methylation found on the differentially methylated regions of imprinted genes, an additional fine tuning of the expressed allele is achieved by a modulation of the H3K9me3 marks, mediated by the association of the H19 lncRNA with chromatin-modifying complexes, such as MBD1. This results in a precise control of the level of expression of growth factors in the embryo.

Tags
Bioruptor
Chromatin Shearing
ChIP-qPCR
Antibody
HighCell ChIP kit
RNA Shearing

Share this article

Published
December, 2013

Source

Products used in this publication

  • ChIP-seq Grade
    C15410039-50
    EZH2 Antibody - ChIP-seq Grade
  • Mouse IgG
    C15410206
    Rabbit IgG
  • Antibody ChIP icon
    C15410078
    MBD1 polyclonal antibody

活动

  • IMB Conference on "Epigenetics of Ageing - Responses to Adversity across Scales" 2022
    Rheinland-Pfalz, Germany
    Jun 28-Jul 1, 2022
  • CNET 2022
    Gif-Sur-Yvette, France
    Jul 4-Jul 6, 2022
 查看所有活动

新闻

 查看所有新闻


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics