Protocols
12 protocols found×
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Download Activity Test - Procedure for immunoprecipitation of native chromatin proteins
Proteins play role in almost all cellular processes, and the study of their activity is crucial for understanding these mechanisms. Slimane AIT-SI-ALI and his “epigenetics and cell fate&rdquo... -
Download ChIP - Just 4 steps to foolproofing fragmentation for ChIP using Bioruptor® Standard, Plus or Pico
Just follow these four steps for shearing chromatin, and you’ll be well on your way to excellent ChIP results without the troubleshooting headache. -
Download ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus
A review of the critical points for chromatin shearing. -
Download ChIP from plant material with the LowCell# ChIP Kit
As preparation of plant material for Chromatin Immunoprecipiation differs essentially from preparation of culture cells, the LowCell# ChIP protocol has to be adapted respectively. We recommend ther... -
Download Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor® Pico
Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in cells and tissues. The assay can be cumbersome and the success is highly dependent on the quality of ... -
Download Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor® Standard, Plus or Pico
This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq ki... -
Download Chromatin Shearing in Yeast Cells with Bioruptor® Standard and Plus
Standard procedure to shear Chromatin from Yeast. -
Download Chromatin shearing with the Bioruptor Pico 10 µl - 300 µl
HeLa cells are fixed with 1% molecular grade formaldehyde (for 8 min at room temperature). Nucleus isolation is performed using buffers and reagents of Diagenode’s Chromatin Shearing Optimiza... -
Download Chromatin shearing with the Bioruptor® Pico 500 µl - 2 ml
Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. ... -
Download Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder
The first critical step of a successful ChIP experiment is the preparation of sheared chromatin with a suitable fragment range which best represents the biological scenario being investigated. The ... -
Download TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®
The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein complex. The fusion of the TAP tag to the protein of interest allows the rapid purification under a nat... -
Download Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®
The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using Bioruptor&...