Chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) is the method of choice to identify, from the whole genome, which specific regions are associated with proteins of interest, like chromatin remodelers or transcription factors.
Traditional ChIP-seq protocols require large amounts of cells, which makes their use to study limited material such as patients samples or embryonic tissues, inaccurate. In order to solve that, Diagenode has combined several high quality tools, to offer the new µChIPmentation for histones protocol for efficient ChIP-seq on 10,000 cells:
An optimized chromatin preparation protocol based on Diagenode’s True MicroChIP technology and shearing in 0.2 ml tubes with Bioruptor Pico
The adaptation of the workflow to use only 3 tubes per sample for the whole process, from cell fixation to purified libraries, to reduce DNA lost
The use of the ChIPmentation technology which enables the integration of the library preparation during the ChIP itself using transposase and sequencing-compatible adaptors for a reduced number of steps
ChIPmentation was developed in collaboration with CeMM in Vienna. The improved protocol of µChIPmentation was developed in collaboration with Robert Månsson and Charlotte Gustafsson at Karolinska Institutet, Sweden.