Diagenode

D-Plex small RNA-seq

19 publications found.

The piRNA-pathway factor FKBP6 is essential for spermatogenesis butdispensable for control of meiotic LINE-1 expression in humans.
Published: October, 2022

Abstract: Infertility affects around 7\% of the male population and can be due to severe spermatogenic failure (SPGF), resulting in no or very few sperm in the ejaculate. We initially identified a homozygous frameshift variant in FKBP6 in a man with extreme...
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The seminal plasma microbiome of men with testicular germ cell tumours described by small RNA sequencing
Published: September, 2022

Abstract: Background: It has been estimated that microorganisms are involved in the pathogenesis of approximately 20% of all cancers. Testicular germ cell tumours (TGCTs) are the most common type of malignancy in young men and arise from the precursor ...
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Neutral sphingomyelinase 2 inhibition attenuates extracellular vesiclerelease and improves neurobehavioral deficits in murine HIV.
Published: July, 2022

Abstract: People living with HIV (PLH) have significantly higher rates of cognitive impairment (CI) and major depressive disorder (MDD) versus the general population. The enzyme neutral sphingomyelinase 2 (nSMase2) is involved in the biogenesis of ceramide ...
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A novel, essential trans-splicing protein connects the nematode SL1snRNP to the CBC-ARS2 complex.
Published: July, 2022

Abstract: Spliced leader trans-splicing is essential for gene expression in many eukaryotes. To elucidate the molecular mechanism of this process, we characterise the molecules associated with the Caenorhabditis elegans major spliced leader snRNP (SL1 snRNP...
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Interspecies effectors of a transgenerational memory of bacterial infection in Caenorhabditis elegans .
Published: July, 2022

Abstract: The inheritance of memory is an adaptive trait. Microbes challenge the immunity of organisms and trigger behavioral adaptations that can be inherited, but how bacteria produce inheritance of a trait is unknown. We use and its bacteria to study the...
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Diverse Monogenic Subforms of Human Spermatogenic Failure
Published: July, 2022

Abstract: Non-obstructive azoospermia (NOA) is the most severe form of male infertility and typically incurable with current medicine. Due to the biological complexity of sperm production, defining the genetic basis of NOA has proven challenging, and to dat...
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Ultrasensitive Ribo-seq reveals translational landscapes duringmammalian oocyte-to-embryo transition and pre-implantation development.
Published: June, 2022

Abstract: In mammals, translational control plays critical roles during oocyte-to-embryo transition (OET) when transcription ceases. However, the underlying regulatory mechanisms remain challenging to study. Here, using low-input Ribo-seq (Ribo-lite), we in...
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Translation is a key determinant controlling the fate of cytoplasmic longnon-coding RNAs
Published: May, 2022

Abstract: Despite predicted to lack coding potential, cytoplasmic long non-coding (lnc)RNAs can associate with ribosomes, resulting in some cases into the production of functional peptides. However, the biological and mechanistic relevance of this pervasive...
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MGcount: a total RNA-seq quantification tool to address multi-mappingand multi-overlapping alignments ambiguity in non-coding transcripts
Published: January, 2022

Abstract: Background Total-RNA sequencing (total-RNA-seq) allows the simultaneous study of both the coding and the non-coding transcriptome. Yet, computational pipelines have traditionally focused on particular biotypes, making assumptions that are not full...
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Single cell quantification of ribosome occupancy in early mouse development
Published: December, 2021

Abstract: Technological limitations precluded transcriptome-wide analyses of translation at single cell resolution. To solve this challenge, we developed a novel microfluidic isotachophoresis approach, named RIBOsome profiling via IsoTachoPhoresis (Ribo-ITP...
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Single cell quantification of ribosome occupancy in early mousedevelopment
Published: December, 2021

Abstract: Technological limitations precluded transcriptome-wide analyses of translation at single cell resolution. To solve this challenge, we developed a novel microfluidic isotachophoresis approach, named RIBOsome profiling via IsoTachoPhoresis (Ribo-ITP...
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Functional microRNA targetome undergoes degeneration-induced shift in the retina
Published: August, 2021

Abstract: Background: MicroRNA (miRNA) play a significant role in the pathogenesis of complex neurodegenerative diseases including age-related macular degeneration (AMD), acting as post-transcriptional gene suppressors through their association with ...
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Functional microRNA targetome undergoes degeneration-induced shift inthe retina.
Published: August, 2021

Abstract: BACKGROUND: MicroRNA (miRNA) play a significant role in the pathogenesis of complex neurodegenerative diseases including age-related macular degeneration (AMD), acting as post-transcriptional gene suppressors through their association with argonau...
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Single-cell microRNA sequencing method comparison and application tocell lines and circulating lung tumor cells
Published: July, 2021

Abstract: Molecular single cell analyses provide insights into physiological and pathological processes. Here, in a stepwise approach, we first evaluate 19 protocols for single cell small RNA sequencing on MCF7 cells spiked with 1 pg of 1,006 miRNAs....
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Small RNAs in Seminal Plasma as Novel Biomarkers for Germ Cell Tumors
Published: May, 2021

Abstract: Circulating miRNAs secreted by testicular germ cell tumors (TGCT) show great potential as novel non-invasive biomarkers for diagnosis of TGCT. Seminal plasma (SP) represents a biofluid closer to the primary site. Here, we investigate whether small...
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Small RNAs in Seminal Plasma as Novel Biomarkers for GermCell Tumors.
Published: May, 2021

Abstract: Circulating miRNAs secreted by testicular germ cell tumors (TGCT) show great potential as novel non-invasive biomarkers for diagnosis of TGCT. Seminal plasma (SP) represents a biofluid closer to the primary site. Here, we investigate whether small...
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Bacterial small RNAs and host epigenetic effectors of atransgenerational memory of pathogens in C. elegans
Published: March, 2021

Abstract: The inheritance of memories is adaptive for survival. Microbes interact with all organisms challenging their immunity and triggering behavioral adaptations. Some of these behaviors induced by bacteria can be inherited although the mechanisms of ac...
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Interspecies RNA Interactome of Pathogen and Host in aHeritable Defensive Strategy.
Published: January, 2021

Abstract: Communication with bacteria deeply impacts the life history traits of their hosts. Through specific molecules and metabolites, bacteria can promote short- and long-term phenotypic and behavioral changes in the nematode . The chronic exposure of to...
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Distinct Extracellular RNA Profiles in Different PlasmaComponents.
Published: January, 2021

Abstract: Circulating extracellular RNAs (exRNAs) have great potential to serve as biomarkers for a wide range of diagnostic, therapeutic, and prognostic applications. So far, knowledge of the difference among different sources of exRNAs is limited. To addr...
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