| Which tubes for which Bioruptor®? QUICK GUIDE Diagenode tubes were developed specifically for use with the Bioruptor®. They ensure a maximu... | Download |
| Datasheet of Bioruptor tubes DATASHEET Datasheet of Diagenode tubes for Bioruptor Pico and Bioruptor Plus. | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: 0.2 ml microtubes for Picoruptor (Diagenode Cat# C30010020). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Targeted bisulfite sequencing for biomarker discovery. |
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new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { 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The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p> <p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p> <p><strong>Application versatility</strong>:</p> <ul> <li>DNA shearing for Next-Generation-Sequencing</li> <li>Chromatin shearing</li> <li>RNA shearing</li> <li>Protein extraction from tissues and cells (also for mass spectrometry)</li> <li>FFPE DNA extraction</li> <li>Protein aggregation studies</li> <li>CUT&RUN - shearing of input DNA for NGS</li> </ul> <div style="background-color: #f1f3f4; margin: 10px; padding: 50px;"> <p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p> </div> <div class="extra-spaced"><img alt="Bioruptor Sonication for DNA shearing for Next-Generation-Sequencing" src="https://www.diagenode.com/img/product/shearing_technologies/guarantie.png" /></div> <div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div> <div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Specifications', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => 'Available shearing Kits', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'bioruptorpico2', 'meta_title' => 'Bioruptor® Pico sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Pico sonication device', 'modified' => '2023-06-20 18:16:03', 'created' => '2020-01-09 16:27:17', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3049', 'antibody_id' => null, 'name' => 'Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'description' => '<p><span>The tube holder for 0.2 ml tubes has been especially designed for being used with the <strong>Bioruptor® Pico</strong> and to guarantee homogeneity of chromatin and DNA shearing. 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Download our quick guide <a href="../documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup><span>®</span></sup>?"</a> to find the best tubes for your applications.</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-pico-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Pico accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Pico', 'meta_title' => 'Accessories for Bioruptor® Pico sonication device | Diagenode', 'modified' => '2018-02-14 16:24:24', 'created' => '2015-06-12 10:01:53', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '791', 'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ', 'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>', 'image_id' => null, 'type' => 'Quick guide', 'url' => 'files/organigram/bioruptor-organigram-tubes.pdf', 'slug' => 'bioruptor-organigram-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:18:51', 'created' => '2015-07-24 16:46:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1074', 'name' => 'Datasheet of Bioruptor tubes', 'description' => '<p>Datasheet of Diagenode tubes for Bioruptor Pico and Bioruptor Plus.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf', 'slug' => 'tds-bioruptor-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:21:44', 'created' => '2020-03-23 10:41:46', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4019', 'name' => 'Targeted bisulfite sequencing for biomarker discovery.', 'authors' => 'Morselli, M and Farrell, C and Rubbi, L and Fehling, HL and Henkhaus, Rand Pellegrini, M', 'description' => '<p>Cytosine methylation is one of the best studied epigenetic modifications. In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development and differentiation and its dysregulation can lead to or is associated with diseases, such as cancer, loss-of-imprinting syndromes and neurological disorders. It has been also shown that DNA methylation at the cellular, tissue and organism level varies with age. To overcome the costs of Whole-Genome Bisulfite Sequencing, the gold standard method to detect 5-methylcytosines at a single base resolution, DNA methylation arrays have been developed and extensively used. This method allows one to assess the status of a fraction of the CpG sites present in the genome of an organism. In order to combine the relatively low cost of Methylation Arrays and digital signals of bisulfite sequencing, we developed a Targeted Bisulfite Sequencing method that can be applied to biomarker discovery for virtually any phenotype. Here we describe a comprehensive step-by-step protocol to build a DNA methylation-based epigenetic clock.</p>', 'date' => '2020-08-02', 'pmid' => 'http://www.pubmed.gov/32755621', 'doi' => '10.1016/j.ymeth.2020.07.006', 'modified' => '2020-12-18 13:27:14', 'created' => '2020-10-12 14:54:59', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptorpico2"><img src="/img/product/shearing_technologies/B01080000-1.jpg" alt="Bioruptor Pico" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01080010</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-3046" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Picoruptor 2 sonication device </strong>. 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columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01201144</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3049" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/3049" id="CartAdd/3049Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3049" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'B01201144', '1850', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'B01201144', '1850', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-2-ml-tube-holder-dock-for-bioruptor-pico" data-reveal-id="cartModal-3049" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> 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In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development and differentiation and its dysregulation can lead to or is associated with diseases, such as cancer, loss-of-imprinting syndromes and neurological disorders. It has been also shown that DNA methylation at the cellular, tissue and organism level varies with age. To overcome the costs of Whole-Genome Bisulfite Sequencing, the gold standard method to detect 5-methylcytosines at a single base resolution, DNA methylation arrays have been developed and extensively used. This method allows one to assess the status of a fraction of the CpG sites present in the genome of an organism. In order to combine the relatively low cost of Methylation Arrays and digital signals of bisulfite sequencing, we developed a Targeted Bisulfite Sequencing method that can be applied to biomarker discovery for virtually any phenotype. 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Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p> <p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p> <p><strong>Application versatility</strong>:</p> <ul> <li>DNA shearing for Next-Generation-Sequencing</li> <li>Chromatin shearing</li> <li>RNA shearing</li> <li>Protein extraction from tissues and cells (also for mass spectrometry)</li> <li>FFPE DNA extraction</li> <li>Protein aggregation studies</li> <li>CUT&RUN - shearing of input DNA for NGS</li> </ul> <div style="background-color: #f1f3f4; margin: 10px; padding: 50px;"> <p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p> </div> <div class="extra-spaced"><img alt="Bioruptor Sonication for DNA shearing for Next-Generation-Sequencing" src="https://www.diagenode.com/img/product/shearing_technologies/guarantie.png" /></div> <div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div> <div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Specifications', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => 'Available shearing Kits', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'bioruptorpico2', 'meta_title' => 'Bioruptor® Pico sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Pico sonication device', 'modified' => '2023-06-20 18:16:03', 'created' => '2020-01-09 16:27:17', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3049', 'antibody_id' => null, 'name' => 'Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'description' => '<p><span>The tube holder for 0.2 ml tubes has been especially designed for being used with the <strong>Bioruptor® Pico</strong> and to guarantee homogeneity of chromatin and DNA shearing. It allows for reproducible sonication of up to<strong> 16 x 0.2 ml tubes</strong> at a time with a <strong>sample volume range of 20 to 100 µl</strong>. The holder is made up of a tube holder and its specific dock that makes the sample preparation more ergonomic. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B01201144', 'old_catalog_number' => '', 'sf_code' => 'B01201144-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '1650', 'price_USD' => '1850', 'price_GBP' => '1500', 'price_JPY' => '258470', 'price_CNY' => '', 'price_AUD' => '4625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => '0-2-ml-tube-holder-dock-for-bioruptor-pico', 'meta_title' => 'Tube holder for 0.2 ml tubes - Bioruptor® Pico', 'meta_keywords' => '', 'meta_description' => 'Tube holder for 0.2 ml tubes - Bioruptor® Pico', 'modified' => '2022-06-09 17:13:58', 'created' => '2020-01-21 11:00:25', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '40', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Pico accessories', 'description' => '<p>All you need for the smooth running of the Bioruptor<sup>®</sup> Pico. Download our quick guide <a href="../documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup><span>®</span></sup>?"</a> to find the best tubes for your applications.</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-pico-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Pico accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Pico', 'meta_title' => 'Accessories for Bioruptor® Pico sonication device | Diagenode', 'modified' => '2018-02-14 16:24:24', 'created' => '2015-06-12 10:01:53', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '791', 'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ', 'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>', 'image_id' => null, 'type' => 'Quick guide', 'url' => 'files/organigram/bioruptor-organigram-tubes.pdf', 'slug' => 'bioruptor-organigram-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:18:51', 'created' => '2015-07-24 16:46:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1074', 'name' => 'Datasheet of Bioruptor tubes', 'description' => '<p>Datasheet of Diagenode tubes for Bioruptor Pico and Bioruptor Plus.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf', 'slug' => 'tds-bioruptor-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:21:44', 'created' => '2020-03-23 10:41:46', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4019', 'name' => 'Targeted bisulfite sequencing for biomarker discovery.', 'authors' => 'Morselli, M and Farrell, C and Rubbi, L and Fehling, HL and Henkhaus, Rand Pellegrini, M', 'description' => '<p>Cytosine methylation is one of the best studied epigenetic modifications. In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development and differentiation and its dysregulation can lead to or is associated with diseases, such as cancer, loss-of-imprinting syndromes and neurological disorders. It has been also shown that DNA methylation at the cellular, tissue and organism level varies with age. To overcome the costs of Whole-Genome Bisulfite Sequencing, the gold standard method to detect 5-methylcytosines at a single base resolution, DNA methylation arrays have been developed and extensively used. This method allows one to assess the status of a fraction of the CpG sites present in the genome of an organism. In order to combine the relatively low cost of Methylation Arrays and digital signals of bisulfite sequencing, we developed a Targeted Bisulfite Sequencing method that can be applied to biomarker discovery for virtually any phenotype. Here we describe a comprehensive step-by-step protocol to build a DNA methylation-based epigenetic clock.</p>', 'date' => '2020-08-02', 'pmid' => 'http://www.pubmed.gov/32755621', 'doi' => '10.1016/j.ymeth.2020.07.006', 'modified' => '2020-12-18 13:27:14', 'created' => '2020-10-12 14:54:59', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptorpico2"><img src="/img/product/shearing_technologies/B01080000-1.jpg" alt="Bioruptor Pico" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01080010</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-3046" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Picoruptor 2 sonication device </strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=3046" id="Quote-3046" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="3046" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="Zi9YTDZEMmhMT1pyNUJseDBnRTZhdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="bioruptorpico2" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 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columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01201144</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3049" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/3049" id="CartAdd/3049Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3049" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'B01201144', '1850', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'B01201144', '1850', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-2-ml-tube-holder-dock-for-bioruptor-pico" data-reveal-id="cartModal-3049" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> 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In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development and differentiation and its dysregulation can lead to or is associated with diseases, such as cancer, loss-of-imprinting syndromes and neurological disorders. It has been also shown that DNA methylation at the cellular, tissue and organism level varies with age. To overcome the costs of Whole-Genome Bisulfite Sequencing, the gold standard method to detect 5-methylcytosines at a single base resolution, DNA methylation arrays have been developed and extensively used. This method allows one to assess the status of a fraction of the CpG sites present in the genome of an organism. In order to combine the relatively low cost of Methylation Arrays and digital signals of bisulfite sequencing, we developed a Targeted Bisulfite Sequencing method that can be applied to biomarker discovery for virtually any phenotype. 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Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p> <p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p> <p><strong>Application versatility</strong>:</p> <ul> <li>DNA shearing for Next-Generation-Sequencing</li> <li>Chromatin shearing</li> <li>RNA shearing</li> <li>Protein extraction from tissues and cells (also for mass spectrometry)</li> <li>FFPE DNA extraction</li> <li>Protein aggregation studies</li> <li>CUT&RUN - shearing of input DNA for NGS</li> </ul> <div style="background-color: #f1f3f4; margin: 10px; padding: 50px;"> <p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p> </div> <div class="extra-spaced"><img alt="Bioruptor Sonication for DNA shearing for Next-Generation-Sequencing" src="https://www.diagenode.com/img/product/shearing_technologies/guarantie.png" /></div> <div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div> <div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Specifications', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => 'Available shearing Kits', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'bioruptorpico2', 'meta_title' => 'Bioruptor® Pico sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Pico sonication device', 'modified' => '2023-06-20 18:16:03', 'created' => '2020-01-09 16:27:17', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3049', 'antibody_id' => null, 'name' => 'Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'description' => '<p><span>The tube holder for 0.2 ml tubes has been especially designed for being used with the <strong>Bioruptor® Pico</strong> and to guarantee homogeneity of chromatin and DNA shearing. 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Download our quick guide <a href="../documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup><span>®</span></sup>?"</a> to find the best tubes for your applications.</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-pico-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Pico accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Pico', 'meta_title' => 'Accessories for Bioruptor® Pico sonication device | Diagenode', 'modified' => '2018-02-14 16:24:24', 'created' => '2015-06-12 10:01:53', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '791', 'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ', 'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>', 'image_id' => null, 'type' => 'Quick guide', 'url' => 'files/organigram/bioruptor-organigram-tubes.pdf', 'slug' => 'bioruptor-organigram-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:18:51', 'created' => '2015-07-24 16:46:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1074', 'name' => 'Datasheet of Bioruptor tubes', 'description' => '<p>Datasheet of Diagenode tubes for Bioruptor Pico and Bioruptor Plus.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf', 'slug' => 'tds-bioruptor-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:21:44', 'created' => '2020-03-23 10:41:46', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4019', 'name' => 'Targeted bisulfite sequencing for biomarker discovery.', 'authors' => 'Morselli, M and Farrell, C and Rubbi, L and Fehling, HL and Henkhaus, Rand Pellegrini, M', 'description' => '<p>Cytosine methylation is one of the best studied epigenetic modifications. In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development and differentiation and its dysregulation can lead to or is associated with diseases, such as cancer, loss-of-imprinting syndromes and neurological disorders. It has been also shown that DNA methylation at the cellular, tissue and organism level varies with age. To overcome the costs of Whole-Genome Bisulfite Sequencing, the gold standard method to detect 5-methylcytosines at a single base resolution, DNA methylation arrays have been developed and extensively used. This method allows one to assess the status of a fraction of the CpG sites present in the genome of an organism. In order to combine the relatively low cost of Methylation Arrays and digital signals of bisulfite sequencing, we developed a Targeted Bisulfite Sequencing method that can be applied to biomarker discovery for virtually any phenotype. Here we describe a comprehensive step-by-step protocol to build a DNA methylation-based epigenetic clock.</p>', 'date' => '2020-08-02', 'pmid' => 'http://www.pubmed.gov/32755621', 'doi' => '10.1016/j.ymeth.2020.07.006', 'modified' => '2020-12-18 13:27:14', 'created' => '2020-10-12 14:54:59', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptorpico2"><img src="/img/product/shearing_technologies/B01080000-1.jpg" alt="Bioruptor Pico" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01080010</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-3046" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Picoruptor 2 sonication device </strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=3046" id="Quote-3046" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="3046" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="Zi9YTDZEMmhMT1pyNUJseDBnRTZhdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="bioruptorpico2" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 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value="GE">Georgia</option> <option value="DE">Germany</option> <option value="GH">Ghana</option> <option value="GI">Gibraltar</option> <option value="GR">Greece</option> <option value="GL">Greenland</option> <option value="GD">Grenada</option> <option value="GP">Guadeloupe</option> <option value="GU">Guam</option> <option value="GT">Guatemala</option> <option value="GG">Guernsey</option> <option value="GN">Guinea</option> <option value="GW">Guinea-Bissau</option> <option value="GY">Guyana</option> <option value="HT">Haiti</option> <option value="HM">Heard Island and McDonald Islands</option> <option value="VA">Holy See (Vatican City State)</option> <option value="HN">Honduras</option> <option value="HK">Hong Kong</option> <option value="HU">Hungary</option> <option value="IS">Iceland</option> <option value="IN">India</option> <option value="ID">Indonesia</option> <option value="IR">Iran, Islamic Republic of</option> <option value="IQ">Iraq</option> <option value="IE">Ireland</option> <option value="IM">Isle of Man</option> <option value="IL">Israel</option> <option value="IT">Italy</option> <option value="JM">Jamaica</option> <option value="JP">Japan</option> <option value="JE">Jersey</option> <option value="JO">Jordan</option> <option value="KZ">Kazakhstan</option> <option value="KE">Kenya</option> <option value="KI">Kiribati</option> <option value="KP">Korea, Democratic People's Republic of</option> <option value="KR">Korea, Republic of</option> <option value="KW">Kuwait</option> <option value="KG">Kyrgyzstan</option> <option value="LA">Lao People's Democratic Republic</option> <option value="LV">Latvia</option> <option value="LB">Lebanon</option> <option value="LS">Lesotho</option> <option value="LR">Liberia</option> <option value="LY">Libya</option> <option value="LI">Liechtenstein</option> <option value="LT">Lithuania</option> <option value="LU">Luxembourg</option> <option value="MO">Macao</option> <option value="MK">Macedonia, Republic of</option> <option 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id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha6649bb8e89a3a"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-3046" class="alert button expand" form="Quote-3046" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { 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value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>'); $("#Quote-3046 #state-3046").parent().parent().show(); } else if (this.value == 'DE') { $("#Quote-3046 #state-3046").replaceWith('<select name="data[Quote][state]" id="state-3046" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-3046 #state-3046").parent().parent().show(); } else { $("#Quote-3046 #state-3046").parent().parent().hide(); $("#Quote-3046 #state-3046").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-3046" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptorpico2" data-reveal-id="quoteModal-3046" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Picoruptor 2 sonication device </h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/0-2-ml-tube-holder-dock-for-bioruptor-pico"><img src="/img/product/shearing_technologies/tube-holder-pico-2.jpg" alt="Bioruptor Pico Tube Holder" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01201144</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3049" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/3049" id="CartAdd/3049Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3049" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'B01201144', '1850', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'B01201144', '1850', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-2-ml-tube-holder-dock-for-bioruptor-pico" data-reveal-id="cartModal-3049" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Tube holder for 0.2 ml tubes - Picoruptor</h6> </div> </div> </li> ' $related = array( 'id' => '3049', 'antibody_id' => null, 'name' => 'Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'description' => '<p><span>The tube holder for 0.2 ml tubes has been especially designed for being used with the <strong>Bioruptor® Pico</strong> and to guarantee homogeneity of chromatin and DNA shearing. It allows for reproducible sonication of up to<strong> 16 x 0.2 ml tubes</strong> at a time with a <strong>sample volume range of 20 to 100 µl</strong>. 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In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development and differentiation and its dysregulation can lead to or is associated with diseases, such as cancer, loss-of-imprinting syndromes and neurological disorders. It has been also shown that DNA methylation at the cellular, tissue and organism level varies with age. To overcome the costs of Whole-Genome Bisulfite Sequencing, the gold standard method to detect 5-methylcytosines at a single base resolution, DNA methylation arrays have been developed and extensively used. This method allows one to assess the status of a fraction of the CpG sites present in the genome of an organism. In order to combine the relatively low cost of Methylation Arrays and digital signals of bisulfite sequencing, we developed a Targeted Bisulfite Sequencing method that can be applied to biomarker discovery for virtually any phenotype. Here we describe a comprehensive step-by-step protocol to build a DNA methylation-based epigenetic clock.</p>', 'date' => '2020-08-02', 'pmid' => 'http://www.pubmed.gov/32755621', 'doi' => '10.1016/j.ymeth.2020.07.006', 'modified' => '2020-12-18 13:27:14', 'created' => '2020-10-12 14:54:59', 'ProductsPublication' => array( 'id' => '4866', 'product_id' => '2952', 'publication_id' => '4019' ) ) $externalLink = ' <a href="http://www.pubmed.gov/32755621" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '3046', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p> <div class="row"> <div class="small-12 medium-8 large-8 columns"><br /> <p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p> </div> <div class="small-12 medium-4 large-4 columns"><center> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </center></div> </div> <p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p> <p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p> <p><strong>Application versatility</strong>:</p> <ul> <li>DNA shearing for Next-Generation-Sequencing</li> <li>Chromatin shearing</li> <li>RNA shearing</li> <li>Protein extraction from tissues and cells (also for mass spectrometry)</li> <li>FFPE DNA extraction</li> <li>Protein aggregation studies</li> <li>CUT&RUN - shearing of input DNA for NGS</li> </ul> <div style="background-color: #f1f3f4; margin: 10px; padding: 50px;"> <p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p> </div> <div class="extra-spaced"><img alt="Bioruptor Sonication for DNA shearing for Next-Generation-Sequencing" src="https://www.diagenode.com/img/product/shearing_technologies/guarantie.png" /></div> <div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div> <div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label1' => 'Specifications', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => 'Available shearing Kits', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'bioruptorpico2', 'meta_title' => 'Bioruptor® Pico sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Pico sonication device', 'modified' => '2023-06-20 18:16:03', 'created' => '2020-01-09 16:27:17', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3049', 'antibody_id' => null, 'name' => 'Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'description' => '<p><span>The tube holder for 0.2 ml tubes has been especially designed for being used with the <strong>Bioruptor® Pico</strong> and to guarantee homogeneity of chromatin and DNA shearing. 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Download our quick guide <a href="../documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup><span>®</span></sup>?"</a> to find the best tubes for your applications.</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-pico-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Pico accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Pico', 'meta_title' => 'Accessories for Bioruptor® Pico sonication device | Diagenode', 'modified' => '2018-02-14 16:24:24', 'created' => '2015-06-12 10:01:53', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '791', 'name' => 'Which tubes for which Bioruptor<sup>®</sup>? ', 'description' => '<p><span>Diagenode tubes were developed specifically for use with the Bioruptor<sup>®</sup>. They ensure a maximum energy delivery to samples with a minimal attenuation of ultrasound intensity. This guide helps you to choose the appropriate tubes for your application of interest and sonication on the Bioruptor<sup>®</sup> Standard, Plus, and Pico. </span></p>', 'image_id' => null, 'type' => 'Quick guide', 'url' => 'files/organigram/bioruptor-organigram-tubes.pdf', 'slug' => 'bioruptor-organigram-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:18:51', 'created' => '2015-07-24 16:46:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1074', 'name' => 'Datasheet of Bioruptor tubes', 'description' => '<p>Datasheet of Diagenode tubes for Bioruptor Pico and Bioruptor Plus.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf', 'slug' => 'tds-bioruptor-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:21:44', 'created' => '2020-03-23 10:41:46', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4019', 'name' => 'Targeted bisulfite sequencing for biomarker discovery.', 'authors' => 'Morselli, M and Farrell, C and Rubbi, L and Fehling, HL and Henkhaus, Rand Pellegrini, M', 'description' => '<p>Cytosine methylation is one of the best studied epigenetic modifications. In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development and differentiation and its dysregulation can lead to or is associated with diseases, such as cancer, loss-of-imprinting syndromes and neurological disorders. It has been also shown that DNA methylation at the cellular, tissue and organism level varies with age. To overcome the costs of Whole-Genome Bisulfite Sequencing, the gold standard method to detect 5-methylcytosines at a single base resolution, DNA methylation arrays have been developed and extensively used. This method allows one to assess the status of a fraction of the CpG sites present in the genome of an organism. In order to combine the relatively low cost of Methylation Arrays and digital signals of bisulfite sequencing, we developed a Targeted Bisulfite Sequencing method that can be applied to biomarker discovery for virtually any phenotype. Here we describe a comprehensive step-by-step protocol to build a DNA methylation-based epigenetic clock.</p>', 'date' => '2020-08-02', 'pmid' => 'http://www.pubmed.gov/32755621', 'doi' => '10.1016/j.ymeth.2020.07.006', 'modified' => '2020-12-18 13:27:14', 'created' => '2020-10-12 14:54:59', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptorpico2"><img src="/img/product/shearing_technologies/B01080000-1.jpg" alt="Bioruptor Pico" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01080010</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-3046" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Picoruptor 2 sonication device </strong>. 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columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01201144</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3049" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/3049" id="CartAdd/3049Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3049" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'B01201144', '1850', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'B01201144', '1850', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="0-2-ml-tube-holder-dock-for-bioruptor-pico" data-reveal-id="cartModal-3049" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Tube holder for 0.2 ml tubes - Picoruptor</h6> </div> </div> </li> ' $related = array( 'id' => '3049', 'antibody_id' => null, 'name' => 'Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'description' => '<p><span>The tube holder for 0.2 ml tubes has been especially designed for being used with the <strong>Bioruptor® Pico</strong> and to guarantee homogeneity of chromatin and DNA shearing. 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In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development and differentiation and its dysregulation can lead to or is associated with diseases, such as cancer, loss-of-imprinting syndromes and neurological disorders. It has been also shown that DNA methylation at the cellular, tissue and organism level varies with age. To overcome the costs of Whole-Genome Bisulfite Sequencing, the gold standard method to detect 5-methylcytosines at a single base resolution, DNA methylation arrays have been developed and extensively used. This method allows one to assess the status of a fraction of the CpG sites present in the genome of an organism. In order to combine the relatively low cost of Methylation Arrays and digital signals of bisulfite sequencing, we developed a Targeted Bisulfite Sequencing method that can be applied to biomarker discovery for virtually any phenotype. Here we describe a comprehensive step-by-step protocol to build a DNA methylation-based epigenetic clock.</p>', 'date' => '2020-08-02', 'pmid' => 'http://www.pubmed.gov/32755621', 'doi' => '10.1016/j.ymeth.2020.07.006', 'modified' => '2020-12-18 13:27:14', 'created' => '2020-10-12 14:54:59', 'ProductsPublication' => array( 'id' => '4866', 'product_id' => '2952', 'publication_id' => '4019' ) ) $externalLink = ' <a href="http://www.pubmed.gov/32755621" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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