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Imaging Chromatin Accessibility by Assay ofTransposase-Accessible Chromatin with Visualization.

Miyanari Yusuke

Chromatin accessibility is one of the fundamental structures regulating genome functions including transcription and DNA repair. Recent technological advantages to analyze chromatin accessibility begun to explore the dynamics of local chromatin structures. Here I describe protocols for Assay of Transposase-Accessible Chromatin with Visualization (ATAC-see), which allows us to analyze subnuclear localization of accessible chromatin and quantify accessible chromatin at single-cell level.

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Tagmentase

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Published
January, 2023

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Products used in this publication

  • Tubes
    C01070010-10
    Tagmentase (Tn5 transposase) - unloaded

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