Where stem cell studies & epigenetics research finally meet
CRISPR/Cas 9 antibody
Validated for immunoblot, IP, and IF
In recent years, studies suggest that the mammalian genome undergoes various epigenetic modifications during development and embryonic stem cell (ESC) differentiation. Understanding the mechanisms that control these epigenetic signals is essential in deciphering stem cell biology and the patterns of gene expression during cellular differentiation. ESC gene expression relies heavily on transcriptional factor networks and epigenetic signals that give stem cells their ability to differentiate into different cell types -- histone modification, histone variants, and DNA methylation. Diagenode provides a full solution for your every epigenetics and stem cell research need including ChIP and ChIP-seq antibodies and kits for low cell amounts, DNA methylation kits and antibodies, and sample processing instruments.
Products for epigenetics studies in stem cell research
ChIP and ChIP-seq kits to decipher the epigenetic landscape of stem cells
- Great for low cell numbers from primary cells and stems cells
- See data from the revolutionary MicroChIP/MicroPlex kit for ChIP-seq with only 10,000 cells
DNA methylation kits for sensitive, fast, and NGS-compatible studies
Antibodies for ChIP, ChIP-seq, histone modifications, DNA methylation, hydroxymethylation, and more
- Premium ChIP-seq grade antibodies
- H3K4me3 and H3K27me3 for bivalent domains, hallmark of ES cell genes regulating differentiation
- Histone modifications antibodies
- Histone methyltransferases e.g. Polycomb proteins (Suz12, EZH2) & SET/MLL proteins (e.g. WDR5)
- Histone demethylases (e.g. JARID2) regulating timing of ES differentiation and maintaining cell type identity
- DNA methylation and hydroxylmethylation antibodies
- 5-hmC and TET2, 5mC, DNA methyl transferases antibodies (DNMT1,2,3)
- Reader methyl-CpG binding protein family (MBD1,2,3,4 MeCP2)
- Histone acetyltransferases (HATs) and histone deacetylases (HDACs) related to ES differentiation and adult stem cell function
Sample prep and processing
- Sonication systems for optimal NGS library preparation, chromatin & DNA shearing, and more!
- Automation systems -- see why IP-Star Automation will simplify your life.
References
Santourlidis S, Wernet P, Ghanjati F, Graffmann N, Springer J, Kriegs C, Zhao X, Brands J, Araúzo-Bravo MJ, Neves R, Koegler G, Uhrberg M, Unrestricted somatic stem cells (USSC) from human umbilical cord blood display uncommitted epigenetic signatures of the major stem cell pluripotency genes., Stem Cell Res,2011-01-01,6,60-9, Abstract
Williams K, Christensen J, Pedersen MT, Johansen JV, Cloos PA, Rappsilber J, Helin K, TET1 and hydroxymethylcytosine in transcription and DNA methylation fidelity., Nature,2011-05-19,473,343-8, Abstract
Iliou MS, Lujambio A, Portela A, Brüstle O, Koch P, Andersson-Vincent PH, Sundström E, Hovatta O, Esteller M Bivalent histone modifications in stem cells poise miRNA loci for CpG island hypermethylation in human cancer., Epigenetics,2011-11-01,6,1344-53, Abstract
Singhal N, Graumann J, Wu G, Araúzo-Bravo MJ, Han DW, Greber B, Gentile L, Mann M, Schöler HR, Chromatin-Remodeling Components of the BAF Complex Facilitate Reprogramming., Cell,2010-06-11,141,943-55, Abstract
Wang K, Chen Y, Chang EA, Knott JG, Cibelli JB., Dynamic Epigenetic Regulation of the Oct4 and Nanog Regulatory Regions during Neural Differentiation in Rhesus Nuclear Transfer Embryonic Stem Cells., Cloning Stem Cells.,2009-12,11,483-96, Abstract
Grandjean V, Gounon P, Wagner N, Martin L, Wagner KD, Bernex F, Cuzin F, Rassoulzadegan M, The miR-124-Sox9 paramutation: RNA-mediated epigenetic control of embryonic and adult growth., Development,2009-11-01,136,3647-55, Abstract
Noer A, Lindeman LC, Collas P, Histone H3 modifications associated with differentiation and long-term culture of mesenchymal adipose stem cells., Stem Cells Dev,2009-06-01,18,725-36, Abstract
Pereira CF, Piccolo FM, Tsubouchi T, Sauer S, Ryan NK, Bruno L, Landeira D, Santos J, Banito A, Gil J, Koseki H, Merkenschlager M, Fisher AG, ESCs require PRC2 to direct the successful reprogramming of differentiated cells toward pluripotency., Cell Stem Cell,2010-06-04,6,547-56, Abstract
Alder O, Lavial F, Helness A, Brookes E, Pinho S, Chandrashekran A, Arnaud P, Pombo A, O'Neill L, Azuara V, Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment., Development,2010-08-01,137,2483-92, Abstract
Sher F, Boddeke E, Olah M, Copray S, Dynamic Changes in Ezh2 Gene Occupancy Underlie Its Involvement in Neural Stem Cell Self-Renewal and Differentiation towards Oligodendrocytes, PLoS ONE,2012-07-12, Abstract
Ruzov A, Tsenkina Y, Serio A, Dudnakova T, Fletcher J, Bai Y, Chebotareva T, Pells S, Hannoun Z, Sullivan G, Chandran S, Hay DC, Bradley M, Wilmut I, De Sousa P, Lineage-specific distribution of high levels of genomic 5-hydroxymethylcytosine in mammalian development., Cell Res,2011-09-01,21,1332-42, Abstract
Stroud H, Feng S, Morey Kinney S, Pradhan S, Jacobsen SE, 5-Hydroxymethylcytosine is associated with enhancers and gene bodies in human embryonic stem cells., Genome Biol,2011-06-20,12,R54, Abstract
Wu H, D'Alessio AC, Ito S, Wang Z, Cui K, Zhao K, Sun YE, Zhang Y, Genome-wide analysis of 5-hydroxymethylcytosine distribution reveals its dual function in transcriptional regulation in mouse embryonic stem cells., Genes Dev,2011-04-01,25,679-84, Abstract
Boulland JL, Mastrangelopoulou M, Boquest AC, Jakobsen R, Noer A, Glover JC, Collas P., Epigenetic Regulation of Nestin Expression During Neurogenic Differentiation of Adipose Tissue Stem Cells., Stem Cells Dev.,2012-12-21, Abstract
Marks H, Kalkan T, Menafra R, Denissov S, Jones K, Hofemeister H, Nichols J, Kranz A, Francis Stewart A, Smith A, Stunnenberg HG, The transcriptional and epigenomic foundations of ground state pluripotency., Cell,2012-04-27,149,590-604, Abstract