Best CRISPR/Cas9 antibodies in the market

 

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The antibody worked very well, even during our first attempt. We did a 1 hour incubation on a shaker at 4 degrees instead of overnight. We wanted to verify expression of Cas9 in various cell lines I had made (immunofluorescence). We have used the antibody a number of times and it works every time.

Researcher at Harvard University

A breakthrough in genome engineering! The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation.

Diagenode offers the widest range of antibodies against CRISPR/Cas9 on the market! These antibodies have been raised against Cas9 from Streptococcus pyogenes and give excellent results in all major applications (immunoblot, immunoprecipitation and immunofluorescence). The perfect tool for monitoring your CRISPR/Cas9 experiments.

 

 

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Highly specific signal in immunofluorescence

HeLa cells were transfected with Cas9 and stained with the CRISPR/Cas9 antibody 4G10 at 4°C overnight, followed by incubation with an anti-mouse secondary antibody coupled to AF488 for 1 hour at room temperature (upper figure). The lower figure shows counter-staining of the nuclei with Hoechst 33342.

 


Clone 4G10 is more efficient in western blot

Western blot was performed on protein extracts from HeLa cells transfected with CRISPR/Cas9 antibody 4G10 using antibodies against Cas9. The antibodies were used at different dilutions (from left to right 1:250, 1:500, 1:1,000 and 1:2,000). The results obtained with the 7A9 clone are shown on the left, the results obtained with the new 4G10 clone are shown on the right.

 

Better performance than tag antibodies

 

Figure 1: Western blot was performed on protein extracts from HEK293 cells transfected with a myc-tagged Cas9 using the Diagenode antibody against CRISPR/Cas9 as well as two commercially available purified monoclonal myc tag antibodies (supplier A and B). The three antibodies were used at different dilutions (antibody stocks: purified monoclonal Myc A 1μg/μl, Myc B 1μg/μl and Cas9 1.9 μg/μl). The ladder has been revealed by using the HRP coupled Blue ladder antibody (C15200202). Exposure time was 1 hour.