Figure 1. Protein Extraction Beads are required for efficient tissue disruption using
the Bioruptor®
Complete disruption is observed in the sample containing Diagenode's Protein Extraction Beads (left) after 5 cycles while non-disrupted tissue is still present in the sample without the Protein Extraction Beads (right).
Figure 2. Total proteins effectively extracted from tissues using Bioruptor® Pico
30 mg of frozen mouse liver were disrupted in RIPA buffer in 1.5 ml Bioruptor® Microtubes with Caps (Cat. No. C30010016) or 15 ml Bioruptor® Tubes (Cat. No. C30010016) supplemented with Protein extraction beads (Cat.No. C20000021) for 3 – 5 cycles with the Bioruptor® Pico (Cat.No. B01060001).
Samples were centrifuged and analyzed by PAGE (Figure A) and Western blot using antibodies against GAPDH (Figure B) and beta tubulin (Figure C).
