Diagenode

ZHX2 polyclonal antibody - Classic

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410260-25
25 μg
$125.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against ZHX2 (zinc fingers and homeoboxes 2), using a recombinant protein.

Lot40107
Concentration0.86 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2-5 μg/ChIP Fig 1, 2
Western Blotting 1:500 - 1:3,000 Fig 3
Immunofluorescence 1:100 - 1:1,000 Fig 4
Immunohistochemistry 1:100 - 1:1,000 Fig 5

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    Figure 1. ChIP results obtained with the Diagenode antibody directed against ZHX2
    ChIP assays were performed using HepG2 cells, the Diagenode antibody against ZHX2 (Cat. No. C15410260) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the CDC25C and AFTPH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against ZHX2
    ChIP was performed on sheared chromatin from 5 million HepG2 cells using 5 μg of the Diagenode antibody against ZHX2 (Cat. No. C15410260). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of chromosomes 1 (fig 2A and B) and in two genomic regions surrounding the CDC25C and AFTPH genes on chromosome 5 and 2, respectively (fig 2C and D).

    Figure 3. Western blot analysis using the Diagenode antibody directed against ZHX2
    Whole cell extracts from HepG2 cells (30 μg) were analysed by Western blot using the Diagenode antibody against ZHX2 (Cat. No. C15410260) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Figure 4. Immunofluorescence with the Diagenode antibody directed against ZHX2
    HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against ZHX2 (Cat. C15410260) diluted 1:500 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain.

    Figure 5. Immunohistochemistry using the Diagenode antibody directed against ZHX2
    Formalin fixed paraffin embedded SAS xenograft tissue was stained with the Diagenode antibody against ZHX2 (Cat. No. C15410260) diluted 1:100 followed by a peroxidase labelled goat anti-rabbit secondary antibody.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet ZHX2 C15410260 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

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Events

  • Workshop on Chromatin Proteomics
    Crete, Greece
    Oct 3-Oct 8, 2016
  • 2nd Annual Next Generation Sequencing Congress
    Boston, USA
    Oct 3-Oct 4, 2016
  • XXXIX Reunión anual de la Sociedad de Bioquimica y Biología Molecular de Chile
    Puerto Varas, Chile
    Sep 27-Sep 30, 2016
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