Diagenode

TRRAP polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410257-100
100 μg
$325.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against TRRAP (transformation/transcription domain-associated protein), using a recombinant protein.

Lot41591
Concentration1 μg/μl
Species reactivityhuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 3
Immunoprecipitation 1:100 - 1:500 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 4, 5
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP results

    Figure 1. TRRAP ChIP results
    ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or TRRAP antibody. The precipitated DNA was detected by PCR with primer set targeting to nucleolin promoter.

    IP results

    Figure 2. TRRAP IP results
    TRRAP antibody immunoprecipitates TRRAP protein in IP experiments. IP samples: 293T whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of TRRAP protein by 4 μg TRRAP antibody (Cat. No. C15410257) 5 % SDS-PAGE The immunoprecipitated TRRAP protein was detected by TRRAP antibody (C15410257) diluted 1:500.

    IF results

    Figure 3. TRRAP IF results
    TRRAP antibody detects TRRAP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRRAP protein stained by TRRAP antibody (Cat. No. C15410257) diluted 1:1,000. Blue: Hoechst 33342 staining.

    Western blot results

    Figure 4. TRRAP Western blot
    results TRRAP antibody detects TRRAP protein by western blot analysis. A. 30 μg Jurkat whole cell extract B. 30 μg Raji whole cell extract C. 30 μg NCI-H929 whole cell extract 5 % SDS-PAGE TRRAP antibody (Cat. No. C15410257) dilution: 1:500.

    Western blot results

    Figure 5. TRRAP western blot
    results TRRAP antibody detects TRRAP protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg A431 whole cell extract C. 30 μg HeLa whole cell extract D. 30 μg HepG2 whole cell extract 5 % SDS-PAGE TRRAP antibody (Cat. No. C15410257) dilution: 1:500.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet TRRAP C15410257 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: TRRAP polyclonal antibody - Classic (Diagenode Cat# C15410257-100 Lot# 41591). Click here to copy to clipboard.

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