Diagenode

THOC1 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410243-25
25 μg
$125.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against THOC (nuclear matrix Protein p84 (THO complex 1), using a recombinant protein.

Lot40163
Concentration1 μg/μl
Species reactivityHuman, mouse
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 3
IHC 1:100 - 1:1,000 Fig 4
Immunoprecipitation 1:100 - 1:500 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 5
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP results

    Figure 1. THOC1 ChIP results
    ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or THOC1 antibody. The precipitated DNA was detected by PCR with primer set targeting to calreticulin.

    IP results

    Figure 2. THOC1 IP results
    THOC1 immunoprecipitates THOC1 (p84) protein in IP experiments. IP Sample: HepG2 whole cell extract A: 30 μg whole cell extract of THOC1 protein expressing HepG2 cells B: Control with 3 μg of pre-immune rabbit IgG C: Immunoprecipitation of THOC1 by 3 μg of THOC1 antibody. The immunoprecipitated THOC1 protein was detected by western blot with the THOC1 antibody diluted 1:1,000.

    IF results

    Figure 3. THOC1 IF results
    THOC1 antibody detects THOC1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: THOC1 protein stained by THOC1 antibody diluted 1:500. Blue: Hoechst 33342 staining.

    IHC results

    Figure 4. THOC1 IHC results
    Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using THOC1 antibody at a 1:500 dilution.

    Western blot results

    Figure 5. THOC1 Western blot results
    Sample: 30 μg of A549 whole cell lysate 7.5% SDS PAGE THOC1 antibody diluted 1:1,000

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet THOC1 C15410243 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: THOC1 polyclonal antibody - Classic (Diagenode Cat# C15410243-25 Lot# 40163). Click here to copy to clipboard.

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