Diagenode

GTF2E2 polyclonal antibody - Classic

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410264-100
100 μg
$325.00
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Other format

Polyclonal antibody raised in rabbit against GTF2E2 (General Transcription Factor IIE Subunit 2), using a recombinant protein.

Lot40723
Concentration1 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP 2-5 μg/ChIP Fig 1, 2
Western Blotting 1:1,000 - 1:10,000 Fig 3
Immunoprecipitation 2.5 μg/IP Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    ChIP results

    Figure 1. ChIP results obtained with the Diagenode antibody directed against GTF2E2
    ChIP assays were performed using HeLa cells, the Diagenode antibody against GTF2E2 (Cat. No. C15410264) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP- seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the GAPDH and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ChIP-seq results figure A

    ChIP-seq results figure B

    ChIP-seq results figure C

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against GTF2E2
    ChIP was performed on sheared chromatin from 4 million HeLa cells using 5 μg of the Diagenode antibody against GTF2E2 (Cat. No. C15410264) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the ChIP-seq signal in three genomic regions surrounding the GAPDH and EIF4A2 positive control genes (fig 2A and B) and the EIF3S2 gene (fig 2C).

    Western blot analysis

    Figure 3. Western blot analysis using the Diagenode antibody directed against GTF2E2
    Whole cell extracts from Jurkat cells (30 μg) were analysed by Western blot using the Diagenode antibody against GTF2E2 (Cat. No. C15410264) diluted 1:5,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Immunoprecipitation

    Figure 4. Immunoprecipitation using the Diagenode antibody directed against GTF2E2
    Immunoprecipitation was performed on whole cell extracts from Jurkat cells using 2.5 μg of the Diagenode antibody against GTF2E2 (Cat. No. C15410264). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated GTF2E2 protein was detected by western blot with the GTF2E2 antibody diluted 1:1,000. The IP with the GTF2E2 antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (30 μg of Jurkat whole cell extract).

  • Applications
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
    ChIP-seq (ab)
    Read more
  • Documents
    Datasheet GTF2E2 polyclonal C15410264 DATASHEET
    Datasheet description
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: GTF2E2 polyclonal antibody - Classic (Diagenode Cat# C15410264-100 Lot# 40723). Click here to copy to clipboard.

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