TAL1 monoclonal antibody - Classic

Catalog Number
50 μg/50 μl
  Bulk order

Monoclonal antibody raised in mouse against human TAL1 (T-Cell Acute Lymphocytic Leukemia 1), using a recombinant protein. 

Concentration1 μg/μl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2-5 μg/ChIP Fig 1, 2

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.

  • Validation data

    ChIP figure 1

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against TAL1
    ChIP was performed using K562 cells, the Diagenode monoclonal antibody against TAL1 (Cat. No. C15200012) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the RUNX1 and RUNX1T1 genes, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ChIP-seq figure 2

    ChIP-seq figure 2

    ChIP-seq figure 2

    ChIP-seq figure 2

    Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against TAL1
    ChIP was performed with 5 μg of the Diagenode antibody against TAL1 (Cat. No. C15200012) on sheared chromatin from 4 million K562 cells as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 2.5 mb region of chromosome 1 (figure 2A and B) and in two regions surrounding the RUNX1and RUNX1T1 positive control genes (figure 2C and D). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.

  • Applications
  • Documents
    TAL1 monoclonal antibody DATASHEET
    Datasheet of the TAL1 monoclonal antibody
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: TAL1 monoclonal antibody - Classic (Diagenode Cat# C15200012 Lot# 001). Click here to copy to clipboard.

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