Diagenode

SMYD3 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410253-25
25 μl
$125.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against SMYD3 (SET and MYND domain containing 3), using a recombinant protein.

Lot42130
Concentration1.32 μg/μl
Species reactivityHuman, mouse, rat
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP* 5 μg/ChIP Fig 1
Western blotting 1:500 - 1:1,000 Fig 2, 3
Immunoprecipitation 5 μg/IP Fig 4
IF 1:500 Fig 5

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against SMYD3
    ChIP was performed on HepG2 cells with 5 μg of the Diagenode antibody against SMYD3 (Cat. No. C15410253). IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the 5’ flanking region of Nkx2.8. Figure 1 shows the fold enrichment over the IgG negative control.

    Immunoprecipitation

    Figure 2. Western blot analysis using the Diagenode antibody directed against SMYD3
    Whole cell extracts from 293T cells (30 μg) transfected with a SMYD3 expression vector (lane 2, partial fragment) and untransfected control cells (lane 1) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Immunofluorescence

    Figure 3. Western blot analysis using the Diagenode antibody directed against SMYD3
    Whole cell extracts (30 μg) from 293T, A431, HeLa and HepG2 cells (lane 1, 2, 3 and 4, respectively) were analysed by western blot using the Diagenode antibody against SMYD3 (Cat. No. C15410253) diluted 1:1.000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Western Blot

    Figure 4. Immunoprecipitation using the Diagenode antibody directed against SMYD3
    Immunoprecipitation was performed on whole cell extracts from 293T cells using 5 μg of the Diagenode antibody against SMYD3 (Cat. No. C15410253) (lane 2) or with an equal amount of rabbit IgG (lane 1). The immunoprecipitated SMYD3 protein was detected by western blot with the SMYD3 antibody diluted 1:1,000.

    Western Blot

    Figure 5. Immunofluorescence using the Diagenode antibody directed against SMYD33
    HCT116 cells were stained with the Diagenode antibody against SMYD3 (Cat. No. C15410253). Cells were fixed with ice-cold methanol for 5 min and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the SMYD3 antibody (left) diluted 1:500 in blocking solution. The right panel shows costaining with Hoechst 33342.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet SMYD3 C15410253 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: SMYD3 polyclonal antibody - Classic (Diagenode Cat# C15410253-25 Lot# 42130). Click here to copy to clipboard.

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