SMAD2 polyclonal antibody - Classic

Catalog Number
100 μg
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Polyclonal antibody raised in rabbit against SMAD2 (SMAD family member 2), using a recombinant protein.

Concentration1 μg/μl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 3
Immunoprecipitation 1:500 - 1:1,000 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 4
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data


    Figure 1. ChIP
    SMAD2 antibody immunoprecipitates SMAD2 protein-DNA in ChIP experiments. ChIP Sample: HeLa chromatin extract A. 5 μg preimmune rabbit IgG B. 5 μg of SMAD2 antibody (C15410251). The precipitated DNA was detected by PCR with primer set targeting to E-cadherin promoter.


    Figure 2. Immunoprecipitation
    SMAD2 antibody immunoprecipitates SMAD2 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell extract A. 50 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of SMAD2 protein by 2 μg of SMAD2 antibody (Cat. No. C15410251). The immunoprecipitated SMAD2 protein was detected by western blot with the SMAD2 antibody diluted 1:1,000.


    Figure 3. Immunofluorescence
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using SMAD2 (Cat. No. C15410251) antibody at a 1:200 dilution.

    Western Blot

    Figure 4. Western Blot
    Sample: 30 μg of Hela whole cell lysate 10% SDS PAGE C15410251 diluted 1:1000

  • Applications
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Immunoprecipitation Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet SMAD2 C15410251 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: SMAD2 polyclonal antibody - Classic (Diagenode Cat# C15410251-100 Lot# 40044). Click here to copy to clipboard.

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