Diagenode

SMAD2 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410251-100
100 μg
$325.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against SMAD2 (SMAD family member 2), using a recombinant protein.

Lot40044
Concentration1 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 3
Immunoprecipitation 1:500 - 1:1,000 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 4
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP

    Figure 1. ChIP
    SMAD2 antibody immunoprecipitates SMAD2 protein-DNA in ChIP experiments. ChIP Sample: HeLa chromatin extract A. 5 μg preimmune rabbit IgG B. 5 μg of SMAD2 antibody (C15410251). The precipitated DNA was detected by PCR with primer set targeting to E-cadherin promoter.

    Immunoprecipitation

    Figure 2. Immunoprecipitation
    SMAD2 antibody immunoprecipitates SMAD2 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell extract A. 50 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of SMAD2 protein by 2 μg of SMAD2 antibody (Cat. No. C15410251). The immunoprecipitated SMAD2 protein was detected by western blot with the SMAD2 antibody diluted 1:1,000.

    Immunofluorescence

    Figure 3. Immunofluorescence
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using SMAD2 (Cat. No. C15410251) antibody at a 1:200 dilution.

    Western Blot

    Figure 4. Western Blot
    Sample: 30 μg of Hela whole cell lysate 10% SDS PAGE C15410251 diluted 1:1000

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet SMAD2 C15410251 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: SMAD2 polyclonal antibody - Classic (Diagenode Cat# C15410251-100 Lot# 40044). Click here to copy to clipboard.

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