Diagenode

SIN3A polyclonal antibody - Classic

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410250-100
100 μg
$325.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against SIN3A (SIN3 Transcription Regulator Family Member A), using a recombinant protein.

Lot41507
Concentration1 μg/μl
Species reactivityHuman, mouse, rat
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2 μg/ChIP Fig 1, 2
Western Blotting 1:500 - 1:3,000 Fig 3
Immunoprecipitation 3 μg/IP Fig 4
Immunofluorescence 1:100 - 1:1,000 Fig 5
Immunohistochemistry 1:100 - 1:1,000 Fig 6

* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    ChIP results

    Figure 1. ChIP results obtained with the Diagenode antibody directed against SIN3A
    ChIP assays were performed using HeLa cells, the Diagenode antibody against SIN3A (Cat. No. C15410250) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the GAPDH and EIF2S3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ChIP-seq results figure A

    ChIP-seq results figure B

    ChIP-seq results figure C

    ChIP-seq results figure D

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SIN3A
    ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against SIN3A (Cat. No. C15410250) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the EIF2S3 and GAPDH positive control genes (fig 2C and D).

    Western blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against SIN3A
    Whole cell extracts from 293T (lane 1), A431 (lane 2), HeLa (lane 3) and HepG2 (lane 4) cells were analysed by Western blot using the Diagenode antibody against SIN3A (Cat. No. C15410250) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Immunoprecipitation

    Figure 4. Immunoprecipitation using the Diagenode antibody directed against SIN3A
    Immunoprecipitation was performed on whole cell extracts from 293T cells using 3 μg of the Diagenode antibody against SIN3A (Cat. No. C15410250). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated SIN3A protein was detected by western blot with the SIN3A antibody diluted 1:1,000. The IP with the SIN3A antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (30 μg of 293T whole cell extract).

    Immunofluorescence

    Figure 5. Immunofluorescence with the Diagenode antibody directed against SIN3A
    HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against SIN3A (Cat. C15410250) diluted 1:1,000 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain.

    Immunohistochemistry

    Figure 6. Immunohistochemistry using the Diagenode antibody directed against SIN3A
    Formalin fixed paraffin embedded mouse prostate (fig 6A), mouse colon (fig 6B) or rat fore brain (fig 6C) tissue was stained with the Diagenode antibody against SIN3A (Cat. No. C15410250) diluted 1:500 followed by a peroxidase labelled goat anti-rabbit secondary antibody.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet SIN3A C15410250 DATASHEET
    Datasheet description
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

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