Diagenode

SETDB1 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410249-25
25 μl
$125.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against SETDB1 (SET domain, bifurcated 1), using a recombinant protein.

Lot40471
Concentration0.63 µg/µl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.

Immunoprecipitation1:100 - 1:500Fig 3

Applications Suggested dilution * References
Western Blotting 1:500 - 1:3,000 Fig 1
Immunoprecipitation 1-100 - 1:500 Fig 2

* Optimal dilutions/concentrations should be determined by the researcher.

  • Validation Data

    Western Blot analysis

    Figure 1. Western Blot analysis using the Diagenode antibody directed against SETDB1
    Whole cell extracts (30 μg) from HeLa cells were analysed by Western blot using the Diagenode antibody against SETDB1 (Cat. No. C15410249) diluted 1:10,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    Immunoprecipitation

    Figure 2. Immunoprecipitation using the Diagenode antibody directed against SETDB1
    Immunoprecipitation was performed on whole cell extracts from HeLa cells using 4 μg of the Diagenode antibody against SETDB1 (Cat. No. C15410249, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated SETDB1 protein was detected by western blot with the SETDB1 antibody diluted 1:1,000.

  • Applications
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet SETDB1 C15410249 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: SETDB1 polyclonal antibody - Classic (Diagenode Cat# C15410249-25 Lot# 40471). Click here to copy to clipboard.

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