Diagenode

SATB1 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410248-25
25 μl
$125.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against human SATB1, using a recombinant protein.

Lot40499
Concentration0.81 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
IHC 1:100 - 1:1,000 Fig 4
Immunoprecipitation 1:100 - 1:500 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 3, 5
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP

    Figure 1. ChIP
    ChIP was performed with Jurkat chromatin extract and 5 μg of either control rabbit IgG or SATB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to BCL2 promoter.

    Immunoprecipitation

    Figure 2. Immunoprecipitation
    SATB1 antibody immunoprecipitates SATB1 protein in IP experiments. IP Sample: 293T whole cell extract. A. Control with 2 μg of preimmune rabbit IgG (Cat. No. C15410206). B. Immunoprecipitation of SATB1 protein by 2 μg of SATB1 antibody 7.5% SDS-PAGE The immunoprecipitated SATB1 protein was detected by SATB1 antibody diluted 1:1,000.

    Western Blot

    Figure 3. Western Blot
    SATB1 antibody detects SATB1 protein by western blot analysis. Jurkat whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (C15410248) diluted 1:500

    Immunohistochemistry

    Figure 4. Immunohistochemistry
    Immunohistochemical analysis of paraffin-embedded Colon, using the SATB1 antibody (C15410248) at a 1:250 dilution.

    Western Blot

    Figure 5. Western Blot
    SATB1 antibody detects SATB1 protein by western blot analysis. THP-1 whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (C15410248) diluted 1:500.

  • Applications
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet SATB1 C15410248 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: SATB1 polyclonal antibody - Classic (Diagenode Cat# C15410248-25 Lot# 40499). Click here to copy to clipboard.

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