Diagenode

Recombinant mouse TDG

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Catalog Number
Format
Price
C23020101
10 µg
$340.00

Active full length recombinant mouse N-terminal His tagged TDG (Thymine-DNA Glycosylase) protein, produced in SF9 insect cells.

  • Product Information

    TDG recombinant protein 

    Other names

    HTDG

    Target description

    TDG (UniProt/Swiss-Prot entry Q13569) belongs to the TDG/mug DNA glycosylase family. It removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. It is also able to remove thymine from C/T and T/T mispairings, although with lower activity. Furrther, TDG plays a key role in active DNA demethylation as it binds to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), but not 5-hydroxymethylcytosine (5hmC), and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine.

    Concentration

    0.5µg/µl

    Specificity 

    Mouse

    Purity

    Purified using FPLC, >95% purity as determined by SDS-PAGE.

    Storage Buffer

    20mM Tris-Cl pH 7.6, 1 mM EDTA, 0.15 M NaCl, 10% glycerol, 0.5 mM PMSF and 1 mM DTT.

    Storage

    Store at-80°C; guaranteed stable for 2 years from date of receipt when stored properly.

    Precautions: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

  • Quality Control

    Figure 1.
    SDS page of the TDG recombinant protein. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  • Testimonials

    The Diagenode TDG recombinant protein (Cat. No. C23020101) is a very good tool. I used it as positive control in western blot analysis and during TDG ELISA activity assays. The protein is really active! Indeed the TDG recombinant protein perfectly works in glycosylase activity assays using marked oligos.

    Dr Francesco Spallotta, Division of Cardiovascular Epigenetics, Goethe University, Frankfurt am Main, Germany
  • Documents
    TDG recombinant protein DATASHEET
    Datasheet of TDG recombinant protein
    Download
  • Publications

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