Diagenode

Recombinant human MeCp2

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Catalog Number
Format
Price
C23010106
10 µg
$340.00

Active full length recombinant human N-terminal His tagged MeCP2 (Methyl CpG Binding Protein 2) protein, produced in SF9 insect cells.

 

  • Product Description

    MeCP2 recombinant protein

    Other names

    AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT

    Target description

    MeCP2 (UniProt/Swiss-Prot entry P51608) is a chromosomal protein with abundant binding sites in the chromatin. It belongs to the family of methyl CpG binding proteins which also comprises MBD1, MBD2, MBD3 and MBD4. MeCP2 can bind specifically to methylated promoters, thereby repressing transcription. This transcriptional repression is mediated through interaction with histone deacetylase and the corepressor SIN3A. MeCP2 also is essential for development. Mutations in MeCP2 are the cause of several types of mental retardation including Rett syndrome, a progressive neurological disorder that causes mental retardation in females and mental retardation syndromic X-linked type 13, and may also be involved in Angelman syndrome and susceptibility to some types of autism.

    Concentration

    0.5µg/µl

    Specificity 

    Human

    Purity

    Purified using FPLC, >95% purity as determined by SDS-PAGE.

    Storage Buffer

    20mM Tris-Cl pH 7.6, 1 mM EDTA, 0.15 M NaCl, 10% glycerol, 0.5 mM PMSF and 1 mM DTT.

    Storage

    Store at-80°C; guaranteed stable for 2 years from date of receipt when stored properly.

    Precautions: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

  • Quality Control

    Figure 1.
    MeCP2 activity assay. A 70 bp methylated DNA (50 ng) was incubated with increasing amounts of MeCP2 (lane 2 - 4) at room temperature for 30 min and immediately analyzed on a native polyacrylamide gel. A negative control (no addition of MeCP2) is shown in lane 1.

  • Documents
    MeCP2 recombinant protein DATASHEET
    Datasheet of MeCP2 recombinant protein
    Download
  • Publications

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