Diagenode

PRMT7 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410247-25
25 μg
$125.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against PRMT7 (protein arginine methyltransferase 7), using a recombinant protein.

Lot40674
Concentration1 μg/μl
Species reactivityHuman, mouse, rat
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 3
IHC 1:100 - 1:1,000 Fig 5, 6, 7
Immunoprecipitation 1:100 - 1:500 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 4
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP

    Figure 1. ChIP
    ChIP was performed with NIH3T3 chromatin extract and 5 μg of either control rabbit IgG or PRMT7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Apex2.

    Immunoprecipitation

    Figure 2. Immunoprecipitation
    PRMT7 antibody immunoprecipitates PRMT7 protein in IP experiments. IP Sample: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 μg of PRMT7 antibody (Cat. No. C15410247) The immunoprecipitated PRMT7 protein was detected by PRMT7 antibody (C15410247) diluted 1:1,000.

    Immunofluorescent analysis

    Figure 3. Immunofluorescent analysis
    PRMT7 antibody detects PRMT7 protein in the cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT7 protein stained by PRMT7 antibody (Cat. No. C15410247) diluted 1:500. Blue: Hoechst 33342 staining.

    Western blot

    Figure 4. Western blot Sample: 30 μg of PC-3 whole cell lysate 7.5% SDS PAGE PMRT7 antibody diluted 1:2,000

    Immunohistochemical analysis

    Figure 5. Immunohistochemical analysis
    Immunohistochemical analysis of paraffin-embedded Hela xenograft, using PRMT7 (C15410247) antibody at a 1:500 dilution.

    Immunohistochemical analysis

    Figure 6. Immunohistochemical analysis
    Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using PRMT7 (C15410247) antibody at a 1:500 dilution.

    Immunohistochemical analysis

    Figure 7. Immunohistochemical analysis
    Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using PRMT7 (C15410247) antibody at a 1:500 dilution.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet PRMT7 C15410247 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PRMT7 polyclonal antibody - Classic (Diagenode Cat# C15410247-25 Lot# 40674). Click here to copy to clipboard.

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