Diagenode

Premium Whole Genome Bisulfite Sequencing (WGBS) kit

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Catalog Number
Format
Price
C02030034
8 rxns
$720.00

The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using Illumina® platforms. It has also been validated for bisulfite-converted library preparation from ChIP-derived samples in order to perform ChIP-Bis-Sequencing. This kit contains specially designed enzymes and buffers needed for genome-wide bisulfite sequencing allowing for studying DNA methylation sites and their role in gene regulation. For Reduced Representation Bisulfite Sequencing (RRBS) experiments use the Diagenode’s Premium RRBS Kit.

Looking for multiplexing? Check out our Premium WGBS indexes

  • Characteristics
    • Genome-wide screening for DNA methylation - Library Preparation of bisulfite-converted DNA from whole genomes
    • Compatible with ChIP-Bis-Seq - Combining ChIP and DNA methylation profiles in one assay
    • Complete kit – Bisulfite conversion reagents and library preparation reagents

    ChIP-Bis-sequencing results

    Figure 1: ChIP-Bis-sequencing results obtained with the Diagenode’s Premium WGBS Kit
    A. Chromatin Immunoprecipitation against the H3K27me3 mark was performed using the Diagenode’s iDeal ChIP-seq Kit for Histones on a Gm12878 human cell line. The library was then prepared using the MicroPlex Library Preparation Kit and sequenced. The peaks represent the distribution of the reads. B. The ChIP’d DNA was then further processed with the Premium WGBS Kit and sequenced. The distribution of the reads is shown in black. The methylated CpGs are shown in red and the unmethylated CpGs in blue. Each peak represents the DNA methylation percentage at one CpG.

  • Applications
    Bisulfite conversion
    Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylate... Read more
  • Documents
    Premium WGBS kit MANUAL
    Whole Genome Bisulfite Sequencing (WGBS) is a single nucleotide resolution technique that a...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Premium Whole Genome Bisulfite Sequencing (WGBS) kit (Diagenode Cat# C02030034). Click here to copy to clipboard.

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