Diagenode

PPARG polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410133
(CS-133-100)
50 μg/47 μl
$295.00
  Bulk order

Alternative names: PPAR-gamma, NR1C3

Polyclonal antibody raised in rabbit against human PPARG (peroxisome proliferator-activated receptor gamma), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.

LotA576-001P
Concentration1.07 µg/µl
Species reactivityHuman, mouse
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 1 μg/ChIP Fig 1
ELISA 1:1,000 Fig 2
Western Blotting 1:2,000 Fig 2, Ref 1

* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against PPARG
    ChIP was performed on macrophages derived from mouse bone marrow using the Diagenode antibody against PPARG (cat. No. CS-133-050) and optimized PCR primer sets for qPCR. Sheared chromatin from 1 million cells and 1 μg of PPARg antibody were used per ChIP experiment. IgG was used as a negative IP control. Figure 1A: recovery, expressed as the % of input, of the PDK4 PPAR response element (RE). Figure 1B: recovery of the FABP4 Adipo PPAR RE in cells treated with RSG, a very strong activating ligand of PPARG, and in untreated cells.

    elisa

    Figure 2. Determination of the titer
    To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PPARG (cat. No. CS-133-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:70,250

    elisa

    Figure 3. Western blot analysis using the Diagenode antibody directed against PPARG
    293T cells were transfected with pNTAP-PPARG and 20 μg of protein extract was analysed by Western blot using the Diagenode antibody against PPARG (cat. No. CS-133-100). The antibody was diluted 1:2,000 in TBS-Tween containing 3% skimmed milk. Figure 2 shows the result of 293T cells transfected with pNTAP-PPARG (lane 1) and of non-transfected cells (lane 2). The position of the protein of interest is indicated on the right the marker (in kDa) is shown on the left.

    chip

    chip

    Figure 4. ChIP results obtained with the Diagenode antibody directed against PPARG
    ChIP was performed on macrophages derived from mouse bone marrow using the Diagenode antibody against PPARG (cat. No. CS-133-050) and optimized PCR primer sets for qPCR. Sheared chromatin from 1 million cells and 1 μg of PPARg antibody were used per ChIP experiment. IgG was used as a negative IP control.
    Figure 4A: recovery, expressed as the % of input, of the PDK4 PPAR response element (RE).
    Figure 4B: recovery of the FABP4 Adipo PPAR RE in cells treated with RSG, a very strong activating ligand of PPARG, and in untreated cells.

  • Applications
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet PPARG CS-133-100 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PPARG polyclonal antibody - Classic (Diagenode Cat# C15410133 Lot# A576-001P). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    STAT6 transcription factor is a facilitator of the nuclear receptor PPARγ-regulated gene expression in macrophages and dendritic cells.
    Szanto A, Balint BL, Nagy ZS, Barta E, Dezso B, Pap A, Szeles L, Poliska S, Oros M, Evans RM, Barak Y, Schwabe J, Nagy L
    Peroxisome proliferator-activated receptor γ (PPARγ) is a lipid-activated transcription factor regulating lipid metabolism and inflammatory response in macrophages and dendritic cells (DCs). These immune cells exposed to distinct inflammatory milieu show cell type specification as a result of altered gene expression...

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