Diagenode

PKNOX1 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410267-25
25 μg
$125.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against PKNOX1 (PBX/knotted 1 homeobox 1), using a recombinant protein.

Lot40870
Concentration1 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
Immunofluorescence 1:100 - 1:1,000 Fig 1
Immunoprecipitation 1:100 - 1:1,000 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 3
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    IF results

    Figure 1. PKNOX1 IF results
    PKNOX1 antibody detects PKNOX1 protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKNOX1 protein stained by PKNOX1 antibody diluted 1:1,000. Blue: Hoechst 33342 staining.

    Immunoprecipitation results

    Figure 2. PKNOX1 Immunoprecipitation results
    PKNOX1 antibody immunoprecipitates PKNOX1 protein in IP experiments. IP Sample: HeLa whole cell extract A. 40 μg HeLa whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PKNOX1 protein by 2 μg of PKNOX1 antibody The immunoprecipitated PKNOX1 protein was detected by western blot with the PKNOX1 antibody diluted 1:1,000.

    Western blot results

    Figure 3. PKNOX1 Western blot results
    Sample: 30 μg of whole cell lysate A: HepG2 B: HepG2 nucleus 10% SDS PAGE PKNOX1 antibody diluted 1:2,000.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet PKNOX1 C15410267 DATASHEET
    Datasheet description
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

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