Diagenode

PAX8 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410246-25
25 μg
$125.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against PAX8 (paired box 8), using a recombinant protein.

Lot39967
Concentration1 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 3
IHC 1:100 - 1:1,000 Fig 4
Immunoprecipitation 1:500 - 1:1,000 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 5
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP

    Figure 1. ChIP
    PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments. ChIP Sample: 293T chromatin extract A. 5 μg preimmune rabbit IgG B. 5 μg of PAX8 antibody (C15410246) The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.

    Immunoprecipitation

    Figure 2. Immunoprecipitation
    PAX8 antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample: 1000 μg 293T whole cell extract A. 40 μg 293T whole cell extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 μg of PAX8 antibody (Cat. No. C15410246). The immunoprecipitated PAX8 protein was detected by western blot with the PAX8 antibody diluted 1:1,000.

    IF results

    Figure 3. IF results
    Immunofluorescence analysis of paraformaldehyde-fixed A549, using PAX8 antibody (Cat. No. C15410246) at a 1:200 dilution.

    Immunohistochemical analysis

    Figure 4. Immunohistochemical analysis
    PAX8 antibody detects PAX8 protein in the nucleus of BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. PAX8 antibody (Cat. No. C15410246) dilution: 1:500.

    Western Blot

    Figure 5. Western Blot
    Sample: 30 μg 293T whole cell lysate 10% SDS PAGE PAX8 antibody diluted 1:1,000

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet PAX8 C15410246 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PAX8 polyclonal antibody - Classic (Diagenode Cat# C15410246-25 Lot# 39967). Click here to copy to clipboard.

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