Diagenode

PARP1 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410245-100
100 μg
$325.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against PARP1 (poly (ADP-ribose) polymerase 1), using a recombinant protein.

Lot41031
Concentration1 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 3
IHC 1:100 - 1:1,000 Fig 4
Immunoprecipitation 1:100-1:500 Fig 6
Western Blotting 1:5,000 - 1:20,000 Fig 2, 5
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP

    Figure 1. ChIP
    ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.

    Western Blot

    Figure 2. Western Blot
    PARP1 antibody detects PARP1 protein by Western blot analysis. A. 30 μg HCT116 whole cell extract (untreated) B. 30 μg HCT116 whole cell extract (30 μM cisplatin treatment for 24hr) 7.5 % SDS-PAGE PARP1 antibody (Cat. No. C15410245) dilution: 1:5,000

    Immunofluorescent analysis

    Figure 3. Immunofluorescent analysis
    PARP1 antibody detects PARP1 protein in the nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP1 protein stained by PARP1 antibody (Cat. No. C15410245) diluted 1:500. Blue: Hoechst 33342 staining.

    Immunohistochemical analysis

    Figure 4. Immunohistochemical analysis
    PARP1 antibody detects PARP1 protein in the nucleus of HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. PARP1 antibody (Cat. No. C15410245) dilution: 1:500.

    Western Blot

    Figure 5. Western Blot
    Sample: 30 μg of whole cell lysate A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE C15410245 dilution 1:10,000

    Immunoprecipitation

    Figure 6. Immunoprecipitation
    PARP1 antibody immunoprecipitates PARP1 protein in IP experiments. IP samples: HCT-116 whole cell extract A. 30 μg HCT-116 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of PARP1 protein with 4 μg PARP1 antibody (Cat. No. C15410245) 5 % SDS-PAGE The immunoprecipitated PARP1 protein was detected by PARP1 antibody (C15410245) diluted 1:500.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet PARP1 C15410245 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PARP1 polyclonal antibody - Classic (Diagenode Cat# C15410245-100 Lot# 41031). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

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