Figure 1. Western blot analysis using the Diagenode antibody directed against hPaf1
A. Nuclear extracts from HeLa cells (HeLa NE, 20 μg) were analysed by western blot using the Diagenode antibody against hPaf1 (cat# CS-015-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
B. A HeLa cell lysate was analysed by Western blot with the hPaf1 antibody diluted 1:2,000. Size and location of the protein are indicated.