Diagenode

PAD4 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410244-25
25 μl
$125.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against PAD4 (peptidyl arginine deiminase, type IV), using a recombinant protein.

Lot40150
Concentration0.73 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 2
IHC 1:100 - 1:1,000 Fig 3
Western Blotting 1:100 - 1:1,000 Fig 4
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP results

    Figure 1. PAD4 antibody ChIP results
    PAD4 antibody immunoprecipitates PAD4 protein-DNA complex in ChIP experiments. ChIP Sample: 293T chromatin extract A. 5 μg preimmune rabbit IgG. B. 5 μg of PAD4 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

    Immunofluorescence

    Figure 2. PAD4 Immunofluorescence
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using PAD4 antibody at a 1:200 dilution.

    Immunohistochemistry

    Figure 3. Immunohistochemistry
    Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PAD4 antibody at a 1:100 dilution.

    Western blot

    Figure 4. Western blot
    Sample: 30 μg of whole cell lysate A: Hela B: Hep G2 7.5% SDS PAGE PAD4 antibody diluted 1:1,000

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet PAD4 C15410244 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PAD4 polyclonal antibody - Classic (Diagenode Cat# C15410244-25 Lot# 40150). Click here to copy to clipboard.

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