PAD4 polyclonal antibody - Classic

Catalog Number
100 μl
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Polyclonal antibody raised in rabbit against PAD4 (peptidyl arginine deiminase, type IV), using a recombinant protein.

Concentration0.73 μg/μl
Species reactivityHuman
PurityAffinity purified
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 2
IHC 1:100 - 1:1,000 Fig 3
Western Blotting 1:100 - 1:1,000 Fig 4
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP results

    Figure 1. PAD4 antibody ChIP results
    PAD4 antibody immunoprecipitates PAD4 protein-DNA complex in ChIP experiments. ChIP Sample: 293T chromatin extract A. 5 μg preimmune rabbit IgG. B. 5 μg of PAD4 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.


    Figure 2. PAD4 Immunofluorescence
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using PAD4 antibody at a 1:200 dilution.


    Figure 3. Immunohistochemistry
    Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PAD4 antibody at a 1:100 dilution.

    Western blot

    Figure 4. Western blot
    Sample: 30 μg of whole cell lysate A: Hela B: Hep G2 7.5% SDS PAGE PAD4 antibody diluted 1:1,000

  • Applications
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Immunohistochemistry Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet PAD4 C15410244 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: PAD4 polyclonal antibody - Classic (Diagenode Cat# C15410244-100 Lot# 40150). Click here to copy to clipboard.

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