NFKB p50 polyclonal antibody - Classic

Catalog Number
100 μl
  Bulk order

Alternative names: NFKB1, KBF1, EBP-1, NFkappaB, NFKB-P105, P105, NF-KB1 

Polyclonal antibody raised in rabbit against NFKB p50 (NFKB1), using a KLH-conjugated synthetic peptide.

Concentrationnot determined
Species reactivityHuman
PurityWhole antiserum
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:5,000 - 1:25,000
Western Blotting 1:500 - 1:2,000 Fig 1
  • Validation Data

    Western Blot

    Figure 1. NFKB p50 antibody western blot results
    Western blot of HeLa cell extract. All incubations were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5’ each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG diluted 1:2,000 for 1 h.

  • Applications
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet NFKBp50 C15310255 DATASHEET
    Datasheet description
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
  • Publications

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