Diagenode

mTOR polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410262-25
25 μg
$125.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against mTOR (mechanistic target of rapamycin (serine/threonine kinase)), using a recombinant protein.

Lot41059
Concentration1 μg/μl
Species reactivityHuman, mouse, rat
TypePolyclonal
PurityProtein A purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/ChIP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 3
IHC 1:100 - 1:1,000 Fig 5, 6
Immunoprecipitation 1:100 - 1:500 Fig 2
Western Blotting 1:500 - 1:3,000 Fig 4, 7, 8
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP results

    Figure 1. mTOR ChIP results
    ChIP was performed with 293T chromatin extract and 5 μg of either control rabbit IgG or mTOR antibody. The precipitated DNA was detected by PCR with primer set targeting to 45S rDNA promoter.

    IP results

    Figure 2. mTOR IP results
    mTOR antibody immunoprecipitates mTOR protein in IP experiments. IP Sample: 293T whole cell extract. A : Control with 3 μg of pre-immune rabbit IgG. B : Immunoprecipitation of mTOR by 3 μg of mTOR antibody (C15410262). The immunoprecipitated mTOR protein was detected by SDS-PAGE with the mTOR antibody (Cat. No. C15410262) diluted 1:1000.

    IF results

    Figure 3. mTOR IF results
    mTOR antibody detects mTOR protein in the cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 100% MeOH for 5 min. Green: mTOR protein stained by mTOR antibody (C15410262) diluted 1:500. Blue: Hoechst 33342 staining.

    Western blot

    Figure 4. mTOR WB results
    mTOR antibody detects mTOR protein by western blot analysis. 50 μg mouse brain extract 5 % SDS-PAGE mTOR antibody (C15410262) dilution: 1:500

    IHC results

    Figure 5. mTOR IHC results
    mTOR antibody detects mTOR protein in the cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. mTOR antibody (C15410262) dilution: 1:500.

    IHC results

    Figure 6. IHC results
    mTOR antibody detects mTOR protein in the cytosol on hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. mTOR antibody (C15410262) dilution: 1:500.

    Western blot

    Figure 7. mTOR WB results
    mTOR antibody detects mTOR protein by western blot analysis. A. 30 μg HeLa whole cell extract (untreated) B. 30 μg HeLa whole cell extract (500 μM CoCl2 treatment for 24 hr) 5 % SDS-PAGE mTOR antibody (C15410262) dilution: 1:1,000

    Western blot

    Figure 8. mTOR WB results
    mTOR antibody detects mTOR protein by western blot analysis. 30 μg Raji whole cell extract 5 % SDS-PAGE mTOR antibody (C15410262) dilution: 1:1,000

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-qPCR (ab)
    Read more
  • Documents
    Datasheet mTOR C15410262 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: mTOR polyclonal antibody - Classic (Diagenode Cat# C15410262-25 Lot# 41059). Click here to copy to clipboard.

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