Diagenode

Micrococcal Nuclease for Chromatin Assembly (500U/ml)

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Catalog Number
Format
Price
C06070001
0.25 ml
$170.00

Micrococcal nuclease or MNase is a 16.9 kDa endonuclease derived from Staphylococcus aureus. It is purified from an E. coli strain expressing an N-terminal 6XHIS tagged micrococcal nuclease.

  • Nuclease description

    Purified protein exhibit an strong endonuclease activity against single-stranded, double-stranded, circular and linear nucleic acids. The enzyme is active in the pH range of 7.0 - 10.0, with optimal activity at pH 9.2 for both RNA and DNA substrates. The rate of cleavage is 30 times greater at the 5’ side of A or T than at G or C and results in the production of mononucleotides and oligonucleotides with terminal 3’-phosphates. MNase is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

  • Publications

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