Diagenode

MCM6 polyclonal antibody - Classic

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410239-100
100 μg
$325.00
  Bulk order
Other format

Polyclonal antibody raised in rabbit against human MCM6, using a recombinant protein.

Lot41521
Concentration1 μg/μl
Species reactivityHuman
TypePolyclonal
PurityAffinity purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution * References
ChIP assay 5 μg/IP Fig 1
ICC/IF 1:100 - 1:1,000 Fig 5
Immunohistochemistry 1:100 - 1:1,000 Fig 3, 4
Immunoprecipitation 1:100 - 1:500 Fig 6
Western Blotting 1:500 - 1:3,000 Fig 2
* Optimal dilutions/concentrations should be determined by the researcher.
  • Validation Data

    ChIP

    Figure 1. MCM6 ChIP results
    ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or MCM6 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

    Western Blot

    Figure 2. MCM6 Western blot results
    MCM6 antibody detects MCM6 protein by western blot analysis. A. 30 μg 293T whole cell extract B. 30 μg A431whole cell extract C. 30 μg HeLa whole cell extract D. 30 μg HepG2 whole cell extract 7.5 % SDS-PAGE MCM6 antibody dilution: 1:1,000

    Immunohistochemical

    Figure 3. MCM6 IHC results
    MCM6 antibody detects MCM6 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. MCM6 antibody dilution: 1:500.

    Immunohistochemical

    Figure 4. MCM6 IHC results
    MCM6 antibody detects MCM6 protein at nucleus on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. MCM6 antibody dilution: 1:500.

    Immunofluorescence

    Figure 5. MCM6 IF results
    MCM6 antibody detects MCM6 protein at nucleus by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM6 protein stained by MCM6 antibody diluted 1:500. Blue: Hoechst 33342 staining.

    Immunoprecipitation

    Figure 6. MCM6 IP results
    MCM6 antibody immunoprecipitates MCM6 protein in IP experiments. IP Sample: 293T whole cell extract A : 30 μg whole cell extract of MCM6 protein expressing 293T cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of MCM6 by 3 μg of MCM6 antibody. The immunoprecipitated MCM6 protein was detected by western blot using the MCM6 antibody diluted 1:1,000.

  • Applications
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    IHC
    Immunohistochemistry Read more
    IP
    Immunoprecipitation Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  • Documents
    Datasheet MCM6 C15410239 DATASHEET
    Datasheet description
    Download
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MCM6 polyclonal antibody - Classic (Diagenode Cat# C15410239-100 Lot# 41521). Click here to copy to clipboard.

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