Diagenode

MagMeDIP kit

Product image
Catalog Number
Format
Price
C02010020
(mc-magme-A10)
10 rxns
$325.00
Other format

Perform MeDIP (Methylated DNA Immunoprecipitation) to estimate DNA methylation status of your sample using the 5-methylcytidine antibody. The development of Diagenode’s MagMeDIP kit has been largely inspired by the reproducibility standards reached using the SX-8G IP-Star® Automated System.

  • Characteristics
    • Generate highly consistent results with internal controls in 24h
    • Minimize error with many reagents in 1 tube
    • Optimized purification (DIB - DNA isolation buffer)
    • Allows direct correlation between IP’d material & methylation status

    MagMeDIP kit validated using Bioruptor

    The MagMeDIP kit protocol has been validated using DNA sheared by sonication using the Bioruptor®. The Kit includes a DNA isolation buffer (DIB) for an extra-fast method to purify your IP’d material (for qPCR analysis). Alternatively, for other applications, e.g. sequencing, linear amplification or microarray, magnetic DNA purification (IPure) can be performed alternatively.

  • Protocols
    MeDIP-sequencing protocol with the MagMedip kit
    MeDIP-sequencing protocol with the MagMedip kit.
    Download
  • Applications
    Methylated DNA immunoprecipitation
    The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP is ... Read more
  • Documents
    MagMeDIP kit MANUAL
    The Diagenode MagMeDIP kit is designed to immunoprecipitate methylated DNA (Methyl DNA IP...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: MagMeDIP kit (Diagenode Cat# C02010020). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Novel regional age-associated DNA methylation changes within human common disease-associated loci
    Bell CG et al.
    BACKGROUND: Advancing age progressively impacts on risk and severity of chronic disease. It also modifies the epigenome, with changes in DNA methylation, due to both random drift and variation within specific functional loci. RESULTS: In a discovery set of 2238 peripheral-blood genome-wide DNA methylomes aged 1...

    Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing
    Amabile A. et al.
    Gene silencing is instrumental to interrogate gene function and holds promise for therapeutic applications. Here, we repurpose the endogenous retroviruses' silencing machinery of embryonic stem cells to stably silence three highly expressed genes in somatic cells by epigenetics. This was achieved by transiently...

    Trichloroethylene-Induced DNA Methylation Changes in Male F344 Rat Liver
    Jiang Y. et al.
    Trichloroethylene (TCE), a common environmental contaminant, causes hepatocellular carcinoma in mice but not in rats. To understand the mechanisms of the species-specific hepatocarcinogenecity of TCE, we examined the methylation status of DNA in the liver of rats exposed to TCE at 0 or 1000 mg/kg b.w. for 5 days usi...

    Dynamic Interplay between the Transcriptome and Methylome in Response to Oxidative and Alkylating Stress
    Deferme L et al.
    In recent years, it has been shown that free radicals not only react directly with DNA but also regulate epigenetic processes such as DNA methylation, which may be relevant within the context of, for example, tumorigenesis. However, how these free radicals impact the epigenome remains unclear. We therefore investiga...

    Efficiency of methylated DNA immunoprecipitation bisulphite sequencing for whole-genome DNA methylation analysis
    Jeong HM et al.
    We compared four common methods for measuring DNA methylation levels and recommended the most efficient method in terms of cost and coverage. MATERIALS & METHODS: The DNA methylation status of liver and stomach tissues was profiled using four different methods, whole-genome bisulphite sequencing (WG-BS), targe...

    Aflatoxin B1 induces persistent epigenomic effects in primary human hepatocytes associated with hepatocellular carcinoma
    Linda Rieswijka, Sandra M.H. Claessena, Otto Bekersc, Marcel van Herwijnena, Daniël H.J. Theunissena, Danyel G.J. Jennena, Theo M.C.M. de Koka, Jos C.S. Kleinjansa,Simone G.J. van Bredaa
    Chronic exposure to aflatoxin B1 (AFB1) has, in certain regions in the world, been strongly associated with hepatocellular carcinoma (HCC) development. AFB1 is a very potent hepatotoxic and carcinogenic mycotoxin which is frequently reported as a food contaminant. Epigenetic modifications provoked by environmental e...

    Paternal B Vitamin Intake Is a Determinant of Growth, Hepatic Lipid Metabolism and Intestinal Tumor Volume in Female Apc1638N Mouse Offspring
    Sabet JA, Park LK, Iyer LK, Tai AK, Koh GY, Pfalzer AC, Parnell LD, Mason JB, Liu Z, Byun AJ, Crott JW
    Background The importance of maternal nutrition to offspring health and risk of disease is well established. Emerging evidence suggests paternal diet may affect offspring health as well. Objective In the current study we sought to determine whether modulating pre-conception paternal B vitamin intake alters intest...

    Regulation of miR-200c/141 expression by intergenic DNA-looping and transcriptional read-through
    Batista L et al.
    The miR-200 family members have been implicated in stress responses and ovarian tumorigenesis. Here, we find that miR-200c/141 transcription is intimately linked to the transcription of the proximal upstream gene PTPN6 (SHP1) in all physiological conditions tested. PTPN6 and miR-200c/141 are transcriptionally co-reg...

    Maternal immune activation induces GAD1 and GAD2 promoter remodeling in the offspring prefrontal cortex
    Labouesse MA et al.
    Maternal infection during pregnancy increases the risk of neurodevelopmental disorders in the offspring. In addition to its influence on other neuronal systems, this early-life environmental adversity has been shown to negatively affect cortical γ-aminobutyric acid (GABA) functions in adult life, including imp...

    DNA methylation profiling: comparison of genome-wide sequencing methods and the Infinium Human Methylation 450 Bead Chip
    Walker DL, Bhagwate AV, Baheti S, Smalley RL, Hilker CA, Sun Z, Cunningham JM
    AIMS: To compare the performance of four sequence-based and one microarray methods for DNA methylation profiling. METHODS: DNA from two cell lines were profiled by reduced representation bisulfite sequencing, methyl capture sequencing (SS-Meth Seq), NimbleGen SeqCapEpi CpGiant(Nimblegen MethSeq), methylated DNA...

    High cortisol in 5-year-old children causes loss of DNA methylation in SINE retrotransposons: a possible role for ZNF263 in stress-related diseases
    Nätt D, Johansson I, Faresjö T, Ludvigsson J, Thorsell A
    BACKGROUND: Childhood stress leads to increased risk of many adult diseases, such as major depression and cardiovascular disease. Studies show that adults with experienced childhood stress have specific epigenetic changes, but to understand the pathways that lead to disease, we also need to study the epigenetic l...

    Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo
    Jiang YZ1, Manduchi E2, Stoeckert CJ Jr3, Davies PF
    BACKGROUND: Atherosclerosis is a heterogeneously distributed disease of arteries in which the endothelium plays an important central role. Spatial transcriptome profiling of endothelium in pre-lesional arteries has demonstrated differential phenotypes primed for athero-susceptibility at hemodynamic sites associat...

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