Diagenode

N6-methyladenosine (m6A) polyclonal antibody - Pioneer

RFX5-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410208
50 μg/42 μl
$195.00
  Bulk order

Polyclonal antibody raised in rabbit against N6-methyladenosine (m6A) conjugated to LPH.

LotA2125-0010
Concentration1.2 µg/µl
Species reactivityHuman, mouse, other (wide range)
TypePolyclonal
PurityProtein G purified
HostRabbit
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
Dot Blotting 1:400 Fig 1
  • Validation Data

    Dot blot

    Figure 1. Dot blot analysis using the Diagenode antibody directed against m6A
    To demonstrate the specificity of the Diagenode antibody against m6A (cat. No. C15410208), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the respective oligo’s were spotted on the membrane. The antibody was diluted 1:400 in PBS-T containing 10 % skimmed milk and 1% BSA. Figure 1 shows a high specificity of the antibody for the oligonucleotide with the N6-methyladenosine modification.

  • Target description

    N6-methyladenosine (m6A) is a modified base which is abundant in mRNA in most eukaryotes but also has been found in tRNA’s, rRNA’s, snRNA’s and in long non-coding RNA’s. Adenosine methylation is catalyzed by m6A methyltransferase, a large protein complex which has a preference for the consensus sequence GGACU. In human, the m6A modification has been identified in more than 7000 genes. It is preferably present around stop codons and in the 3’ UTR but has not been observed in poly A tails. m6A is dynamically regulated both throughout development and in response to cellular stimuli. Levels are significantly higher in adulthood than during embryonic development.

    Although the presence of m6A in RNA was identified several years ago, it’s physiological significance remains largely unknown. It has been proposed to affect mRNA processing and export from nucleus to cytoplasm. Recently, it was shown that mutations in the m6A demethylase gene FTO, which cause a decrease of m6A levels, are associated with an increased risk for obesity and type 2 diabetes.

     

  • Applications
    DB
    Dot blotting Read more
  • Documents
    Datasheet m6A C15410208 DATASHEET
    Datasheet description
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    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
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    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: N6-methyladenosine (m6A) polyclonal antibody - Pioneer (Diagenode Cat# C15410208 Lot# A2125-0010). Click here to copy to clipboard.

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