LowCell# ChIP kit protein A x16

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16 rxns
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The Diagenode’s LowCell# ChIP kit is the only complete magnetic bead based ChIP (Chromatin Immunoprecipitation) kit that can be used with as low as 1000 - 10 000 cells per IP. Ideal for Stem cells, embryos and biopsies.

  • Characteristics
    • Use from 1,000 - 100,000 cells per IP
    • Improved handling and reproducibility by the use of magnetic beads and special Magnetic rack (0.2 ml tubes)
    • Optimized purification and de-crosslinking step
    • One day from cell collection to results

    The LowCell# ChIP kit has been validated using chromatin sheared by sonication using the Bioruptor®. The Kit includes a DNA purifying slurry for an extra-fast method to purify your IP’d material (for qPCR analysis). Alternatively, for other applications, e.g. sequencing, linear amplification or microarray, magnetic DNA purification (IPure) can be performed alternatively.

  • Protocols
    ChIP from plant material with the LowCell# ChIP Kit
    As preparation of plant material for Chromatin Immunoprecipiation differs essentially from prepar...
    ChIP from tissues with the LowCell# ChIP kit
    In theory, you could use 30 mg of tissue per antibody per ChIP. You will start by optimizing cond...
  • Applications
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
  • Documents
    LowCell# ChIP kit MANUAL
    Diagenode provides kits with optimized reagents and simplified protocols for ChIP includi...
    Chromatin Immunoprecipitation Brochure BROCHURE
    Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: LowCell# ChIP kit protein A x16 (Diagenode Cat# C01010070). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Genomic Characterization of Metformin Hepatic Response
    Luizon M.R. et al.
    Metformin is used as a first-line therapy for type 2 diabetes (T2D) and prescribed for numerous other diseases. However, its mechanism of action in the liver has yet to be characterized in a systematic manner. To comprehensively identify genes and regulatory elements associated with metformin treatment, we carried o...

    DNA demethylation of inflammasome-associated genes is enhanced in patients with cryopyrin-associated periodic syndromes
    Vento-Tormo R et al.
    BACKGROUND: Inflammasomes are cytosolic multiprotein complexes in macrophages. They assemble after infection- or stress-associated stimuli, activating both caspase-1-mediated inflammatory cytokine secretion and pyroptosis. Increased inflammasome activity resulting from gene mutations is related to monogenic autoinf...

    Molecular mechanisms in H2O2-induced increase in AT1 receptor gene expression in cardiac fibroblasts: a role for endogenously generated Angiotensin II.
    Anupama V et al.
    The AT1 receptor (AT1R) mediates the manifold actions of Angiotensin II in the cardiovascular system. This study probed the molecular mechanisms that link altered redox status to AT1R expression in cardiac fibroblasts. Real-time PCR and western blot analysis showed that H2O2 enhances AT1R mRNA and protein expression...

    Epigenetic alterations of CYP19A1 gene in Cumulus cells and its relevance to infertility in endometriosis
    Hosseini E et al.
    PURPOSE: The purpose of the present study was to investigate the epigenetic mechanisms responsible for the aberrant aromatase expression (CYP19A1) in Cumulus Cells (CCs) of infertile endometriosis patients. METHOD: Cumulus cells were obtained from 24 infertile patients with and without endometriosis who underwe...

    Loss of the transcription factor Meis1 prevents sympathetic neurons target-field innervation and increases susceptibility to sudden cardiac death
    Bouilloux F. et al.
    Although cardio-vascular incidents and sudden cardiac death (SCD) are among the leading causes of premature death in the general population, the origins remain unidentified in many cases. Genome-wide association studies have identified Meis1 as a risk factor for SCD. We report that Meis1 inactivation in the mouse ne...

    Analysis of EZH2: micro-RNA network in low and high grade astrocytic tumors
    Vikas Sharma, Suvendu Purkait, Sonam Takkar, Prit Benny Malgulwar, Anupam Kumar, Pankaj Pathak, Vaishali Suri, Mehar C. Sharma, Ashish Suri, Shashank Sharad Kale, Ritu Kulshreshtha, Chitra Sarkar
    Enhancer of Zeste homologue2 (EZH2) is an epigenetic regulator that functions as oncogene in astrocytic tumors, however, EZH2 regulation remains little studied. In this study, we measured EZH2 levels in low (Gr-II,DA) and high grade (Gr-IV,GBM) astrocytic tumors and found significant increased EZH2 transcript level ...

    Molecular basis and functional significance of Angiotensin II-induced increase in Discoidin Domain Receptor 2 gene expression in cardiac fibroblasts
    George M et al.
    Delineation of mechanisms underlying the regulation of fibrosis-related genes in the heart is an important clinical goal as cardiac fibrosis is a major cause of myocardial dysfunction. This study probed the regulation of Discoidin Domain Receptor 2 (DDR2) gene expression and the regulatory links between Angiotensin ...

    An Insulator Element Located at the Cyclin B1 Interacting Protein 1 Gene Locus Is Highly Conserved among Mammalian Species
    Yoshida W, Tomikawa J, Inaki M, Kimura H, Onodera M, Hata K, Nakabayashi K
    Insulators are cis-elements that control the direction of enhancer and silencer activities (enhancer-blocking) and protect genes from silencing by heterochromatinization (barrier activity). Understanding insulators is critical to elucidate gene regulatory mechanisms at chromosomal domain levels. Here, we focused on ...

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